汕头大学医学院学报2026，Vol.39Issue(1)：31-37,7.DOI:10.13401/j.cnki.jsumc.2026.01.006
基于免疫球蛋白G N-糖链特征的结直肠腺瘤诊断模型构建与验证
Construction and validation of a diagnostic model for colorectal adenoma based on immunoglobulin G N-glycan features
摘要
Abstract
Objective:To explore the potential application value of immunoglobulin G(IgG)N-glycan features as biomarkers for the diagnosis of colorectal adenoma(CRA).Methods:A case-control study was conducted,enrolling 90 CRA patients confirmed by colonoscopy and 90 healthy controls from the Second Affiliated Hospital of Shantou University Medical College between March and September 2021.The relative abundance of plasma IgG N-glycans was detected using hydrophilic interaction ultra-performance liquid chromatography(HILIC-UPLC).Characteristic glycans were screened by LASSO regression,and independent associated factors were identified by multivariate logistic regression to construct a nomogram diagnostic model.The discriminative ability and calibration of the model were evaluated using the area under the receiver operating characteristic(ROC)curve(AUC)and calibration curves,respectively.Model stability was validated by the Bootstrap method(1 000 resamples).Results:Among the 22 directly detected IgG N-glycans,12 showed differential abundance between the two groups(adjusted P<0.05 by the Benjamini-Hochberg method).LASSO regression screened six glycans,and multivariate analysis confirmed that GP2(OR=19.00,95%CI:3.32-162.74),GP10(OR=2.24,95%CI:1.18-4.59),GP11(OR=2.33×10-3,95%CI:1.10×10-4-2.37×10-2),and GP19(OR=3.79,95%CI:1.75-9.08)were independently associated with CRA(all P<0.05).The diagnostic model constructed based on these four factors demonstrated good discriminative performance(AUC=0.952,95%CI:0.923-0.980).The average AUC from Bootstrap validation was 0.954(95%CI:0.919-0.981).The calibration curve indicated good consistency between predicted probabilities and actual observations(Brier score=0.088).Conclusion:This study preliminarily confirms that IgG N-glycan features have potential for discriminating CRA.However,due to limitations such as the retrospective design and single-center small sample size,the generalizability and clinical applicability of this diagnostic model require further validation through multicenter prospective studies.关键词
IgG N-糖基化/结直肠腺瘤/诊断模型/列线图/生物标志物Key words
IgG N-glycosylation/colorectal adenoma/diagnostic model/nomogram/biomarker分类
医药卫生引用本文复制引用
许丽丽,郭国湖,陈晓东,许锐锐..基于免疫球蛋白G N-糖链特征的结直肠腺瘤诊断模型构建与验证[J].汕头大学医学院学报,2026,39(1):31-37,7.基金项目
汕头市医疗卫生科技计划(汕府科[2022]81号-85) （汕府科[2022]81号-85）
