天津中医药2026，Vol.43Issue(4)：485-490,6.DOI:10.11656/j.issn.1672-1519.2026.04.11
基于Sirt1/PGC-1α通路探讨豆甾醇对糖尿病肾病大鼠足细胞自噬的影响
Exploring the effect of stigmasterol on autophagy of podocytes in rats with diabetic kidney disease based on the Sirt1/PGC-1α pathway
摘要
Abstract
[Objective]To discuss the effect of stigmasterol on autophagy of podocytes in diabetic kidney disease(DKD)rats based on the silent information regulator 1(Sirt1)/peroxisome proliferator-activated receptor γ coactivator 1α(PGC-1α)pathway.[Methods]DKD rats were established using high-glucose and high-fat,streptozotocin solution,and stochastically separated into the DKD group,different doses of stigmasterol(stigmasterol-low50 mg/kg,stigmasterol-high 200 mg/kg)groups,valsartan(8.33 mg/kg)group,and stigmasterol-high+EX527(5 mg/kg)group.And the rats fed with ordinary feed were regarded as the normal group.The urine protein content and blood glucose were detected.The levels of blood urea nitrogen(BUN)and serum creatinine(Scr)were detected by the kits.Renal tissues were isolated to evaluate histopathology and changes in podocyte structure and autophagosomes.The positive expressions of podocular pore membrane protein molecules(Nephrin,Podocin),the expressions of autophagy marker proteins(Beclin-1,P62)mRNA,and the expressions of Sirt1/PGC-1α pathway-related proteins in renal tissue were detected.[Results]The DKD group had lower positive expressions of Nephrin and Podocin,and the expressions of Beclin-1 mRNA,Sirt1,and PGC-1α proteins,and higher blood glucose,urine protein,Scr,BUN,and expression of P62 mRNA than the normal group(P<0.05).The stigmasterol-low group,stigmasterol-high group and valsartan group had higher positive expressions of Nephrin and Podocin,and the expressions of Beclin-1 mRNA,Sirt1,and PGC-1α proteins,and lower blood glucose,urine protein,Scr,BUN,and expression of P62 mRNA than the DKD group(P<0.05).The stigmasterol-high+EX527 group had lower positive expressions of Nephrin and Podocin,and the expressions of Beclin-1 mRNA,Sirt1,and PGC-1α proteins,and higher blood glucose,urine protein,Scr,BUN,and expression of P62 mRNA than the stigmasterol-high group(P<0.05).[Conclusion]Stigmasterol enhances autophagy of podocytes in DKD rats and alleviates podocyte injury by activating the Sirt1/PGC-1α pathway.关键词
豆甾醇/Sirt1/PGC-1α通路/糖尿病肾病/足细胞/自噬Key words
stigmasterol/Sirt1/PGC-1α pathway/diabetic kidney disease/podocytes/autophagy分类
医药卫生引用本文复制引用
胡冬怡,谷东风,刘变玲,赵继芳,沈蓓莉,吴梦..基于Sirt1/PGC-1α通路探讨豆甾醇对糖尿病肾病大鼠足细胞自噬的影响[J].天津中医药,2026,43(4):485-490,6.基金项目
河南省医学科技攻关计划项目(LHGJ20240889) （LHGJ20240889）
郑州市医疗卫生领域科技创新指导计划项目(2024YLZDJH279). （2024YLZDJH279）
