天津中医药大学学报2026，Vol.45Issue(3)：301-307,7.DOI:10.11656/j.issn.1673-9043.2026.03.07
冬凌草甲素调节IL-6/STAT3信号通路对氧糖剥夺/复氧小胶质细胞增殖、自噬、凋亡及炎症反应的影响
The effect of oridonin regulating the IL-6/STAT3 signaling pathway on proliferation,autophagy,apoptosis,and inflammatory response in microglia subjected to oxygen-glucose deprivation/reoxygenation
摘要
Abstract
[Objective]To investigate the effect of oridonin(ORI)on proliferation,autophagy,apoptosis,and inflammatory response in microglia subjected to oxygen-glucose deprivation/reoxygenation(OGD/R)and its potential mechanism involving the interleukin-6(IL-6)/signal transducer and activator of transcription 3(STAT3)signaling pathway.[Methods]BV2 microglial cells were divided into the following groups:control,OGD/R,ORI(low,medium,and high concentration:ORI-L,ORI-M,ORI-H),and ORI combined with recombinant IL-6.After respective treatments for 24 h,cell proliferation was assessed by Cell Counting Kit-8(CCK-8)assay and 5-ethynyl-2′-deoxyuridine(EdU)staining.Autophagy was observed under a transmission electron microscope.Apoptosis was detected by flow cytometry.The levels of inflammatory factors[tumor necrosis factor-α(TNF-α),IL-18,and IL-1β]were measured using enzyme-linked immunosorbent assay(ELISA).The protein expression levels of IL-6 and STAT3 were determined by Western blotting.[Results]Compared with the control group,the OGD/R group showed decreased cell viability and EdU-positive cell rate,along with increased number of autophagic vacuoles,apoptosis rate,levels of TNF-α,IL-18,IL-1β,and protein expression of IL-6 and STAT3(P<0.01).Compared with the OGD/R group,the ORI-L,ORI-M,and ORI-H groups exhibited a concentration-dependent increase in cell viability and EdU-positive cell rate,and a concentration-dependent decrease in the number of autophagic vacuoles,apoptosis rate,levels of TNF-α,IL-18,IL-1β,and protein expression of IL-6 and STAT3(P<0.05).Compared with the ORI-H group,the ORI combined with recombinant IL-6 group demonstrated decreased cell viability and EdU-positive cell rate,and increased number of autophagic vacuoles,apoptosis rate,levels of TNF-α,IL-18,IL-1β,and protein expression of IL-6 and STAT3(P<0.01).[Conclusion]ORI can promote proliferation and inhibit autophagy,apoptosis,and inflammatory response in OGD/R-induced microglia.Its mechanism may be associated with the inhibition of the IL-6/STAT3 signaling pathway.关键词
冬凌草甲素/白细胞介素-6/转录活化因子3信号通路/氧糖剥夺/复氧/小胶质细胞/增殖/自噬/凋亡Key words
oridonin/IL-6/STAT3 signaling pathway/oxygen-glucose deprivation/reoxygenation/microglia/proliferation/autophagy/apoptosis分类
医药卫生引用本文复制引用
李林,李向男,杨松涛,李建民,张云鹤,付爱军,张志勇..冬凌草甲素调节IL-6/STAT3信号通路对氧糖剥夺/复氧小胶质细胞增殖、自噬、凋亡及炎症反应的影响[J].天津中医药大学学报,2026,45(3):301-307,7.基金项目
河北省医学科学研究课题计划项目(20240045). （20240045）
