应用化学2026，Vol.43Issue(3)：396-410,15.DOI:10.19894/j.issn.1000-0518.250344
基于液相色谱-串联质谱技术的阿胶粉蛋白组分分析及其抗氧化活性评价
Proteomic Analysis and the Antioxidant Activity Evaluation of Asini Corii Colla Powder Based on Liquid Chromatography-Tandem Mass Spectrometry Technology
摘要
Abstract
Asini corii colla contains multiple bioactive proteins,endowing it with multifaceted health benefits.This study employed liquid chromatography-tandem mass spectrometry(LC-MS/MS)to systematically identify and analyze the protein composition of Asini corii colla powder,identifying various functional proteins including multiple intermediate filament proteins,collagens,immunomodulatory proteins,and antioxidant-related proteins.Further evaluation of Asini corii colla powder's cytotoxicity,proliferation,and migration effects was conducted using human dermal fibroblasts(HSF).Results indicated no significant cytotoxicity within the tested concentration range(0.156～20 mg/mL),while promoting HSF proliferation and migration,suggesting preliminary potential for Asini corii colla powder in skin repair applications.To investigate the antioxidant stress activity of Asini corii colla,this study further employed a hydrogen peroxide(H2O2)induced cellular oxidative stress model to evaluate the effects of Asini corii colla powder on intracellular reactive oxygen species(ROS)scavenging capacity and antioxidant enzyme activity.Experiments demonstrated that pre-treatment with Asini corii colla powder significantly suppressed H2O2-induced ROS levels while enhancing intracellular superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-Px)activities.This confirms its antioxidant effects are associated with strengthening the endogenous antioxidant defense system.Proteomics analysis further revealed that Asini corii colla powder may regulate oxidative stress by modulating the expression of proteins associated with the heat shock protein B family,biliverdin reductase B,hemoglobin subunit a,and nuclear factor KB(NF-KB)signaling pathways,thereby mitigating cellular oxidative stress damage to a certain extent.In summary,this study elucidates the protein composition of Asini corii colla and validates its role and mechanisms in combating oxidative stress,laying a scientific foundation for expanding its applications in skin repair and anti-aging.关键词
阿胶粉/蛋白质组学/活性氧清除/氧化应激Key words
Asini corii colla/Proteomics/Reactive oxygen species scavenge/Oxidative stress分类
化学化工引用本文复制引用
解晓,李聪,张建岭,陈智杰,陶春香,扶美梅,尤惠琳,郭金山..基于液相色谱-串联质谱技术的阿胶粉蛋白组分分析及其抗氧化活性评价[J].应用化学,2026,43(3):396-410,15.基金项目
东阿阿胶健康产业科技有限公司横向项目(No.DEEJ-YB-2024-10-190)资助 Supported by the Industry-sponsored Project with Dong'e Ejiao Health Products Co.,Ltd.(No.DEEJ-YB-2024-10-190) （No.DEEJ-YB-2024-10-190）
