中风与神经疾病杂志2026，Vol.43Issue(3)：254-258,5.DOI:10.19845/j.cnki.zfysjjbzz.2026.0044
高铜负荷对大鼠神经行为和SIRT3/PGC-1α信号通路的影响
Influence of high copper load on neurobehavior and the sirtuin 3/peroxisome proliferator-activated receptor-γ co-activator-1α signaling pathway in rats
摘要
Abstract
Objective To investigate the influence of high copper load on neurobehavior in rats and its possible mechanism of action based on the deacetylase sirtuin 3(SIRT3)/peroxisome proliferator-activated receptor-γ coactivator-1α(PGC-1α)signaling pathway.Methods A total of 50 adult Sprague-Dawley rats were randomly divided into model group and blank group,with 25 rats in each group.The rats in the model group were fed with 1 g/kg high-copper diet and 0.185%copper sulfate solution(prepared using deionized water)to establish a rat model of high copper load,and after 12 weeks,the shuttle box test and the pole-climbing test were used to observe neurobehavioral changes,while Western blotting and immunofluorescent staining were used to measure the protein expression levels of SIRT3 and PGC-1α in hippo-campal tissue.Results The shuttle box test showed that compared with the blank group,the model group had a signifi-cant reduction in the number of times of active avoidance reaction after stimulation(P<0.01)and significant increases in the times of active avoidance reaction and passive avoidance reaction(P<0.01);the pole-climbing test showed that com-pared with the blank group,the model group had a significant increase in pole-climbing time(P<0.01).Western blotting and immunofluorescent staining showed that compared with the blank group,the model group had significant reductions in the protein expression levels of SIRT3 and PGC-1α in hippocampal tissue(P<0.05).Conclusion High copper load has a marked influence on the neurobehavior of rats,possibly by regulating the expression of the SIRT3/PGC-1α signal path-way in hippocampal neurons.关键词
肝豆状核变性/大鼠神经行为/SIRT3/PGC-1α/信号通路Key words
Hepatolenticular degeneration/Neurobehavior in rats/Sirtuin 3/peroxisome proliferator-activated receptor-γ coactivator-1α/Signaling pathway分类
医药卫生引用本文复制引用
姜姗,金珊,方向,王洋洋,高婷..高铜负荷对大鼠神经行为和SIRT3/PGC-1α信号通路的影响[J].中风与神经疾病杂志,2026,43(3):254-258,5.基金项目
安徽省自然科学基金面上项目(2008085MH264、2208085MH271) （2008085MH264、2208085MH271）
国家中医药管理局第六批全国老中医药专家学术经验继承项目(国中医药人教发[2017]年29号) （国中医药人教发[2017]年29号）
