中草药2026，Vol.57Issue(6)：2009-2015,7.DOI:10.7501/j.issn.0253-2670.2026.001
反柄紫芝化学成分及其生物活性研究
Chemical constituents isolated from Ganoderma cochlear and their biological activities
摘要
Abstract
Objective To investigate the chemical constituents from the methanol extract of the polyporaceae fungus Ganoderma cochlear and preliminarily evaluate their anti-atherosclerotic activity in vitro.Methods The extract was systematically isolated and purified by D-101 macroporous adsorption resin,silica gel column chromatography,reversed-phase C18 column chromatography,preparative thin layer chromatography and semi-preparative HPLC.The structures of the isolated compounds were elucidated by HR-ESI-MS,IR,and NMR.Meanwhile,the absolute configurations of the new compounds were determined by NMR and ECD calculation.The in vitro anti-atherosclerotic activities of the isolates were assessed using an oxidized low-density lipoprotein(ox-LDL)-induced macrophage lipid deposition model.Results Two novel compounds were isolated and identified from G.cochlear,designated as 7,10-epoxy-3,4-seco-9(10→19)abeo-25,26,27-trinorlanosta-4,11-dioxo-8-en-3,24-dimethyl ester(1)and 6-hydroxy-2-(4-methylpent-3-en-1-yl)-4H-chromen-4-one(2).In the ox-LDL-induced macrophage model,compounds 1 and 2 did not exhibit significant anti-lipid deposition activity at 40 μmol/L.Conclusion This study successfully isolated and identified two new compounds,and named as ganodeconoid J(1)and ganodeconoid K(2).Compound 1 represents the first Ganoderma triterpenoid possessing a 5β-H,enriching the chemical diversity of Ganoderma fungi.Compounds 1 and 2 did not show significant in vitro anti-atherosclerotic activity.关键词
反柄紫芝/三萜/杂萜/反柄紫芝萜J/反柄紫芝萜K/灵芝三萜/脂质沉积Key words
Ganoderma cochlear(Blume&T.Nees)Bres./triterpenoids/meroterpenoids/ganodeconoid J/ganodeconoid K/Ganoderma triterpenoid/lipid deposition分类
医药卫生引用本文复制引用
叶家文,褚天骄,熊利,王欣叶,苏海国,熊亮..反柄紫芝化学成分及其生物活性研究[J].中草药,2026,57(6):2009-2015,7.基金项目
国家自然科学基金项目(82204226) （82204226）
