中草药2026，Vol.57Issue(6)：2022-2028,7.DOI:10.7501/j.issn.0253-2670.2026.06.003
海南产对叶百部中1个新的降倍半萜
A new norsesquiterpenoid from Stemona tuberosa in Hainan
摘要
Abstract
Objective To investigate the chemical components and anti-inflammatory activities of the roots of Stemona tuberosa from Hainan province.Methods The chemical constituents of S.tuberosa were isolated and purified using chromatography techniques such as silica gel,gel column chromatography,and semi-preparative high-performance liquid chromatography(HPLC).Subsequently,the structures of the purified compounds were identified by modern spectroscopic methods such as nuclear magnetic resonance(NMR)and mass spectrometry(MS).Finally,the anti-inflammatory activities of the compounds were evaluated using the lipopolysaccharide(LPS)-induced murine monocyte-macrophage RAW264.7 cell model.Results A total of seven compounds were isolated from S.tuberosa,and identified as(4R)-3,5,5-trimethyl-4-(3-methylbut-2-enoyl)cyclohex-2-en-1-one(1),oxystemofoline(2),methyl 4-hydroxycinnamate(3),(-)-stemoamide(4),tuberostemospiroline(5),stemanthrene C(6),stilbostemin F(7).Compounds 2 and 3 exhibited inhibitory activity against NO production in LPS-induced RAW264.7 cells,with median inhibition concentration(IC50)values of(42.99±4.96)and(70.22±0.81)μmol/L,respectively.Conclusion Compound 1 was identified as a new compound,named tuberostemoterpenone.Compound 2 was isolated from S.tuberosa for the first time.Compounds 2 and 3 exhibited no significant cytotoxicity within the tested concentration range and demonstrated certain anti-inflammatory activity.关键词
对叶百部/生物碱/降倍半萜/抗炎活性/对叶百部萜酮/羟基百部叶碱/4-羟基肉桂酸甲酯Key words
Stemona tuberosa Lour./alkaloids/norsesquiterpenoids/anti-inflammatory activity/tuberostemoterpenone/oxystemofoline/methyl 4-hydroxycinnamate分类
医药卫生引用本文复制引用
朱雄波,黄圣卓,王若仲,许瑞华,万博文,吴妃,魏艳梅,蔡彩虹,王昊,戴好富,梅文莉..海南产对叶百部中1个新的降倍半萜[J].中草药,2026,57(6):2022-2028,7.基金项目
海南省重点研发项目(ZDYF2024SHFZ063) （ZDYF2024SHFZ063）
国家自然科学基金面上项目(82373763) （82373763）
国家热带农业科学中心科技创新团队(CATASCXTD202521) （CATASCXTD202521）
