中草药2026，Vol.57Issue(6)：2060-2074,15.DOI:10.7501/j.issn.0253-2670.2026.06.006
基于金属有机骨架材料IRMOF-10的重楼皂苷VII靶向纳米粒制备、表征及抗三阴性乳腺癌药效评价
Preparation and characterization of polyphyllin VII targeted nanoparticles based on metal-organic framework materials IRMOF-10 and preliminary evaluation of anti-triple negative breast cancer efficacy
摘要
Abstract
Objective To prepare hyaluronic acid-modified polyphyllin VII-loaded metal-organic framework material IRMOF-10 targeted nanoparticles(HA@IRMOF-10@PPVII),systematically evaluate their physicochemical properties,safety and in vitro anti-triple negative breast cancer(TNBC)activity.Methods Metal-organic framework material IRMOF-10 was synthesized by triethylamine method.PPVII was encapsulated by the heterotopic encapsulation method,and the surface of the drug-loaded nanoparticles was modified with hyaluronic acid to construct the targeted nanoparticles HA@IRMOF-10@PPVII.The drug loading was determined by HPLC.The morphology,structure and thermal stability of nanoparticles were characterized by scanning electron microscopy(SEM),Fourier transform infrared spectroscopy(FTIR),thermogravimetric analysis(TG)and BET specific surface area method.Its blood safety was evaluated by hemolysis test,and its cell targeting was verified by laser confocal microscopy.CCK-8,DAPI staining,Annexin V/PI double staining,migration assay,clone formation assay,cell uptake,reactive oxygen species(ROS)detection,mitochondrial membrane potential(MMP)detection and other methods were used to verify its inhibitory effect on TNBC 4T1 cells.Results The drug loading capacity of HA@IRMOF-10@PPVII was(39.56%±1.78)%(n=3).HA@IRMOF-10@PPVII had good biosafety,which could significantly reduce the hemolysis risk of PPVII.This nanoparticle can effectively improve the uptake of PPVII in 4T1 cells,and significantly inhibits cell migration and clone formation.Its anti-tumor mechanism is closely related to inducing an increase in intracellular ROS levels,a decrease in MMP triggering apoptosis,and inhibiting the migration and cloning of 4T1 cells.Conclusion The HA@IRMOF-10@PPVII nanoparticles were successfully prepared,which exhibit excellent targeting ability and anti-TNBC activity.They can significantly enhance the efficacy of PPVII,providing experimental evidence for the development of PPVII-based anti-tumor formulations.关键词
重楼皂苷VII/金属有机骨架材料/IRMOF-10/抗肿瘤/三阴性乳腺癌Key words
polyphyllin VII/metal organic framework materials/IRMOF-10/anti-tumor/triple negative breast cancer分类
医药卫生引用本文复制引用
林舒婷,董晓旭,曲昌海,倪健,尹兴斌,尹东阁,朱荣玥,陈红月,荆霄鸿,王雯琪,常榕蓉,孙宇菲,邓兮玺..基于金属有机骨架材料IRMOF-10的重楼皂苷VII靶向纳米粒制备、表征及抗三阴性乳腺癌药效评价[J].中草药,2026,57(6):2060-2074,15.基金项目
北京市自然科学基金项目(7262198) （7262198）
国家自然科学基金项目(81703715) （81703715）
