中草药2026，Vol.57Issue(6)：2109-2119,11.DOI:10.7501/j.issn.0253-2670.2026.06.010
经典名方桂枝甘草汤基准样品的HPLC指纹图谱及量质传递规律研究
HPLC fingerprint chromatogram and quantity-quality transmitting rule of benchmark samples of classical prescription Guizhi Gancao Decoction
摘要
Abstract
Objective The HPLC fingerprint and the content determination method of the benchmark sample of Guizhi Gancao Decoction(GGD,桂枝甘草汤)were established to study the rule of quantity-quality transmitting of GGD benchmark samples.Methods According to the method recorded in"Treatise on Febrile Diseases",15 batches of GGD benchmark samples were prepared and the HPLC fingerprint of 15 batches of benchmark samples was established.The peak attribution and similarity range in the fingerprint were clarified,and the quantity-quality transfer indexes such as the range of extract yield,the content range of index components and the range of transfer rate were analyzed.The quality control system for GGD benchmark samples was preliminarily established.Results A total of 40 common peaks were identified in the 15 batches of GGD benchmark samples,which with good similarity(>0.99).The contents of protocatechuic acid,liquiritin,coumarin,cinnamyl alcohol,liquiritigenin,cinnamic acid,cinnamaldehyde,and glycyrrhizic acid in the benchmark sample were 0.080-0.087,0.863-1.055,0.431-0.560,0.167-0.227,0.087-0.127,0.423-0.492,0.160-0.199,and 2.437-2.904 mg/g,respectively.The transfer rates of the eight index components from decoction pieces to water decoction were 44.35%—54.84%,40.56%—44.44%,48.82%—56.00%,22.84%—52.61%,25.70%—30.02%,33.75%—37.83%,23.42%—28.98%,and 62.09%—65.13%,respectively.The transfer rates from the water decoction to the reference sample were 67.99%—84.39%,62.15%—67.93%,59.42%—74.22%,76.20%—85.64%,51.31%—60.73%,78.80%—85.17%,16.27%—22.19%,and 71.82%—76.58%,respectively.Overall,the transfer rates of components from decoction pieces to water decoction were relatively low,while the transfer rates of most components increased during the subsequent concentration and freeze-drying processes.Only cinnamaldehyde showed a significant loss during freeze-drying.The dry paste yield of the reference sample was stable,with an average transfer rate of 77.71 %.Conclusion Through the HPLC fingerprint of GGD benchmark samples and the quantity-quality transfer patterns of its active components,a preliminary quality control system based on index components was established.This provides a scientific basis for the standardization and quality consistency evaluation of GGD classical prescription preparations.关键词
经典名方/桂枝甘草汤/指纹图谱/量质传递/基准样品/原儿茶酸/甘草苷/香豆素/肉桂醇/甘草素/肉桂酸/肉桂醛/甘草酸Key words
classical formula/Guizhi Gancao Decoction/fingerprints/quantity-quality transfer/benchmark samples/protocatechuic acid/liquiritin/coumarin/cinnamyl alcohol/liquiritigenin/cinnamic acid/cinnamaldehyde/glycyrrhizic acid分类
医药卫生引用本文复制引用
陈嘉豪,王鹤,晏文轲,董佳琪,廉娜娜,邹俊波,史亚军,栾飞..经典名方桂枝甘草汤基准样品的HPLC指纹图谱及量质传递规律研究[J].中草药,2026,57(6):2109-2119,11.基金项目
国家级大学生创新创业训练计划项目(202410716036) （202410716036）
陕西省中医药管理局中医药科研项目(SZY-KJCYC-2025-JC-043) （SZY-KJCYC-2025-JC-043）
陕西省教育厅重点科学研究计划项目——高校工程研究中心类(25JR059) （25JR059）
陕西中医药大学科技创新人才体系建设计划项目(2024-CXTD-03) （2024-CXTD-03）
教育部代谢性心血管疾病医药基础研究创新中心开放课题基金资助(xnykdxcxzx-2024-06) （xnykdxcxzx-2024-06）
