中国光学（中英文）2026，Vol.19Issue(1)：190-204,15.DOI:10.37188/CO.EN-2025-0025
基于人工智能的高精度菌落提取与分离系统
Artificial intelligence-enabled high-precision colony extraction and isolation system
摘要
Abstract
Standard bacterial suspensions play a crucial role in microbiological diagnosis.Traditional prepar-ation methods,which rely heavily on manual operations,face challenges such as poor reproducibility,low ef-ficiency,and biosafety concerns.In this study,we propose a high-precision automated colony extraction and separation system that combines large-field imaging and artificial intelligence(AI)to facilitate intelligent screening and localization of colonies.Firstly,a large-field imaging system was developed to capture high-resolution images of 90 mm Petri dishes,achieving a physical resolution of 13.2 μm and an imaging speed of 13 frames per second.Subsequently,AI technology was employed for the automatic recognition and localiza-tion of colonies,enabling the selection of target colonies with diameters ranging from 1.9 to 2.3 mm.Next,a three-axis motion control platform was designed,accompanied by a path planning algorithm for the efficient extraction of colonies.An electronic pipette was employed for accurate colony collection.Additionally,a bacterial suspension concentration measurement module was developed,incorporating a 650 nm laser diode as the light source,achieving a measurement accuracy of 0.01 McFarland concentration(MCF).Finally,the system's performance was validated through the preparation of an Esckerichia coli(E.coli)suspension.After 17 hours of cultivation,E.coli was extracted four times,achieving the target concentration set by the system.This work is expected to enable rapid and accurate microbial sample preparation,significantly reducing de-tection cycles and alleviating the workload of healthcare personnel.关键词
人工智能/菌落提取与分离/大视场成像/自动化Key words
artificial intelligence/colony extraction and isolation/large-field imaging/automation分类
医药卫生引用本文复制引用
赵旭峰,贾志强,陈维学,胡鹏涛,苏新然,李俊霖,葛明锋,董文飞..基于人工智能的高精度菌落提取与分离系统[J].中国光学（中英文）,2026,19(1):190-204,15.基金项目
国家重点研发项目(No.2022YFC2406200) （No.2022YFC2406200）
中国科学院科研仪器设备研制项目(No.YJKYYQ20200038) （No.YJKYYQ20200038）
中国科学院重点部署项目(No.YJKYYQ20210032)Supported by National Key R&D Program of China(No.2022YFC2406200) （No.YJKYYQ20210032）
Scientific Instrument and Equip-ment Development Projects of Chinese Academy of Sciences(No.YJKYYQ20200038) （No.YJKYYQ20200038）
Key Deployment Project of Chinese Academy of Sciences(No.YJKYYQ20210032) （No.YJKYYQ20210032）
