中国实验方剂学杂志2026，Vol.32Issue(6)：19-27,9.DOI:10.13422/j.cnki.syfjx.20260119
基于TLR4/NF-κB p65/NLRP3信号通路探讨当归芍药散改善糖尿病肾病小鼠炎症反应机制
Mechanism of Danggui Shaoyaosan in Improving Inflammatory Response in Mice with Diabetic Kidney Disease Based on TLR4/p65/NLRP3 Signaling Pathway
摘要
Abstract
Objective:To investigate the effect of Danggui Shaoyaosan on the expression of Toll-like receptor 4/nuclear factor-kappa B p65/NOD-like receptor protein 3(TLR4/NF-κB p65/NLRP3)signaling pathway in the renal tissues of db/db mice with spontaneous diabetes,and to explore the potential mechanism by which Danggui Shaoyaosan alleviates inflammation in diabetic kidney disease(DKD).Methods:Thirty db/db mice were divided into five groups:A model group,Danggui Shaoyaosan low-(16.77 g·kg-1·d-1),medium-(33.54 g·kg-1·d-1),and high-dose(67.08 g·kg-1·d-1)intervention groups,as well as an irbesartan group(0.025 g·kg-1·d-1)by the random number table method,with 6 mice in each group.Additionally,6 db/m mice were assigned to the normal group.After 8 weeks of intervention,the following parameters were determined by corresponding methods:body weight,fasting blood glucose(FBG),24-hour urinary protein(24 h-UTP),and serum creatinine(SCr)levels,renal histopathological analysis by hematoxylin-eosin(HE)staining,Masson staining,and periodic acid-Schiff(PAS)staining,the protein and mRNA expression levels of TLR4,NF-κB p65,NLRP3,tumor necrosis factor-alpha(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),interleukin-10(IL-10),and interleukin-18(IL-18)by Western blot and Real-time quantitative polymerase chain reaction(Real-time PCR),as well as TLR4,NF-κB p65,and NLRP3 protein expression in renal tissues by immunohistochemistry(IHC).Results:Compared with the normal group,the model group exhibited increased body weight,FBG,24 h-UTP,and SCr levels(P＜0.05);disordered renal structure,thickened basement membrane,and interstitial inflammatory cell infiltration,elevated TLR4,NF-κB p65,NLRP3,TNF-α,IL-1β,IL-6,and IL-18 expression;as well as decreased IL-10 expression(P＜0.05).Compared with the model group,these pathological changes and biochemical abnormalities were reversed in the medicine intervention groups to varying degrees(P＜0.05).Conclusion:Danggui Shaoyaosan may delay DKD progression by alleviating renal inflammatory response and reducing urinary protein excretion via modulating the TLR4/NF-κB p65/NLRP3 signaling pathway.关键词
糖尿病肾病/当归芍药散/炎症反应/Toll受体4/核转录因子-κB二聚体基因/NOD样蛋白受体3通路/db/db小鼠Key words
diabetic kidney disease/Danggui Shaoyaosan/inflammatory response/Toll-like receptor 4/nuclear factor-kappa B p65/NOD-like receptor protein 3(TLR4/NF-κB p65/NLRP3)signaling pathway/db/db mice分类
医药卫生引用本文复制引用
郭世龙,李睿嘉,王子轩,王新爱,侯露瑜,石文婧,田萌媛,郭登洲..基于TLR4/NF-κB p65/NLRP3信号通路探讨当归芍药散改善糖尿病肾病小鼠炎症反应机制[J].中国实验方剂学杂志,2026,32(6):19-27,9.基金项目
河北省中医药管理局科研计划项目(2025285) （2025285）
河北省自然科学基金项目(H2022423367) （H2022423367）
河北中医药大学研究生创新项目(XCXZZSS2025024) （XCXZZSS2025024）
