中国比较医学杂志2026，Vol.36Issue(6)：1-10,10.DOI:10.3969/j.issn.1671-7856.2026.06.001
十枣汤诱导乳腺癌裸鼠移植瘤细胞凋亡的作用及机制研究
Effect and mechanism of Shizao decoction on inducing apoptosis in breast cancer transplants in nude mice
摘要
Abstract
Objective To investigate the effect and underlying mechanism of Shizao decoction(SZD)on inducing apoptosis in breast cancer transplants in nude mice.Methods SKBR3 cells were cultured and transplanted into nude mice to construct tumor models.SZD was applied by continuous intragastric administration for 4 weeks and its effects on tumor growth were observed.After administration,the mice were killed and the transplanted tumor tissues were separated.Lactate dehydrogenase(LDH)levels in tumor tissues were detected using an LDH kit.Morphological changes in transplanted tumor tissues were observed by Hoechst33342-propidium iodide staining,reactive oxygen species(ROS)were evaluated by 2',7'-dichlorodihydrofluorescein diacetate staining,and mitochondrial membrane potential was evaluated by JC-1 assay.Caspase3,Caspase8,and Caspase9 activities in the transplanted tumor cells were detected by enzyme-linked immunosorbent assay.Fas and Fas ligand(FasL)protein expression were detected by Western blot.Caspase3,Caspase8,Caspase9,Fas,and FasL gene expression were detected by quantitative polymerase chain reaction.Results Compared with the model group,SZD significantly increased LDH production(P＜0.05,P＜0.01)by transplanted breast cancer cells in nude mice,induced apoptosis,increased the ROS content(P＜0.05)and mitochondrial depolarization(P＜0.05,P＜0.01),increased the gene expression and enzyme activities of Caspase3,Caspase8,and Caspase9(P＜0.05,P＜0.01),and increased the gene/protein expression of Fas and FasL(P＜0.05,P＜0.01).Conclusions SZD can induce apoptosis in transplanted breast tumors,possibly via the apoptotic caspase and Fas/FasL pathways tumor.关键词
十枣汤/乳腺癌癌/裸鼠/细胞凋亡/凋亡Caspase途径/Fas/FasL途径Key words
Shizao decoction/breast cancer/nude mice/apoptosis/apoptotic caspase pathway/Fas/FasL pathway分类
医药卫生引用本文复制引用
马立威,陈哲,李旭,刘吉成..十枣汤诱导乳腺癌裸鼠移植瘤细胞凋亡的作用及机制研究[J].中国比较医学杂志,2026,36(6):1-10,10.基金项目
国家自然科学基金(82174207) （82174207）
黑龙江省博士后科研启动基金(LBH-QY24005) （LBH-QY24005）
齐齐哈尔医学院重点学科建设项目(QYZDXK-001). （QYZDXK-001）
