中国比较医学杂志2026，Vol.36Issue(6)：51-68,18.DOI:10.3969/j.issn.1671-7856.2026.06.006
程序性死亡基因组结合TCGA数据库筛选CD19和CD79A作为肺腺癌微环境重塑和预后因子的初步研究
Preliminary study on selection of CD19 and CD79A as factors for lung adenocarcinoma microenvironment remodeling and prognosis using the programmed cell death genome in combination with The Cancer Genome Atlas database
摘要
Abstract
Objective Lung adenocarcinoma(LUAD)has a poor prognosis.This study aimed to screen core genes associated with the tumor microenvironment(TME)and programmed cell death(PCD)to provide new prognostic markers and therapeutic targets for LUAD,and to validate their cross-species conservation.Methods Based on RNA-seq data for LUAD and normal lung tissue in The Cancer Genome Atlas(TCGA)database,we assessed the TME and screened for differentially expressed genes(DEGs)using the ESTIMATE algorithm.Functional enrichment analysis(Gene Ontology/Kyoto Encyclopedia of Genes and Genomes),protein-protein interaction(PPI)networks,and univariate Cox regression analysis were performed on the DEGs.Cross-screening combining PPI networks,univariate Cox regression,and the PCD genome was employed to identify core prognostic genes,and their prognostic value and TME association were validated by survival analysis,gene set enrichment analysis(GSEA),and immune infiltration(CIBERSORT)analysis.Results High immune/ESTIMATE scores were significantly associated with prolonged patient survival.The selected shared DEGs were primarily enriched in immune-related pathways.Cross-analysis identified CD19 and CD79A as core prognostic genes associated with PCD.Clinical-feature analysis showed that CD19 and CD79A expression were significantly higher in LUAD tumors than in normal lung tissues,but their expression levels decreased significantly with advancing TNM staging,closely associated with advanced staging,distant metastasis,and poor prognosis.Patients with high CD19/CD79A expression had significantly longer overall survival than those with low expression.Animal-model validation confirmed the cross-species conserved role of CD19/CD79A in tumor progression.GSEA indicated that the high-CD19/CD79Aexpression group was significantly enriched in immune activation pathways(e.g.,allograft rejection,complement response),while the low-expression group was enriched in metabolic(glycolysis,oxidative phosphorylation)and oncogenic pathways.CIBERSORT analysis confirmed that their expression levels were significantly positively correlated with TME immune activity.Conclusions CD19 and CD79A are overexpressed in LUAD tumor tissues,but their expression levels decrease with disease progression.High expression of CD19/CD79A is closely associated with a favorable prognosis and immune-activated TME,while low expression suggests a poor prognosis and immune suppression/oncogenic states.As genes associated with PCD,CD19/CD79A may serve as potential protective prognostic biomarkers and immunotherapeutic targets in LUAD,thereby providing a basis for understanding the immune mechanisms of LUAD and guiding the development of B cell-targeted therapeutic strategies.关键词
肺腺癌/肿瘤微环境/程序性细胞死亡/CD19/CD79A/预后标志物Key words
lung adenocarcinoma/tumor microenvironment/programmed cell death/CD19/CD79A/prognostic markers分类
医药卫生引用本文复制引用
虞家恒,褚云起,洪超金,林幸子,徐圣霞,刘月环..程序性死亡基因组结合TCGA数据库筛选CD19和CD79A作为肺腺癌微环境重塑和预后因子的初步研究[J].中国比较医学杂志,2026,36(6):51-68,18.基金项目
浙江省大学生创新训练计划项目(S202513023001). （S202513023001）
