中国防痨杂志2026，Vol.48Issue(4)：504-509,6.DOI:10.19982/j.issn.1000-6621.20250412
金胺O荧光染色全自动显微扫描识别系统在结核病诊断中的应用价值
Clinical value of auramine O fluorescence staining automatic microscopic scanning and recognition system for detecting tuberculosis
摘要
Abstract
Objective:To evaluate the application value of a fully automatic auramine O fluorescence staining microscopic scanning system(OF-AS,software recognition)in tuberculosis diagnosis.Methods:A total of 503 consecutive patients with suspected pulmonary tuberculosis who visited the outpatient department of Tianjin Tuberculosis Control Center for the first time from January to August 2025 were enrolled.Three initial sputum specimens were collected from each patient,with a total of 1509 specimens which all were subjected to auramine O fluorescence staining,and then microscopic examination was performed with double-blinded method using traditional manual microscopy(abbreviated as"manual microscopy")and OF-AS respectively.One sputum specimen of good quality was selected from each patient for BACTEC MGIT 960 mycobacterial liquid culture(abbreviated as"MGIT 960 culture"),with a total of 503 cases completed.Taking MGIT 960 culture as the gold standard for the detection of pulmonary tuberculosis,the efficacies of manual microscopy and OF-AS in testing sputum specimens from patients with pulmonary tuberculosis were analyzed;rank correlation of positive results detected by both manual microscopy and OF-AS was analyzed.Results:The positive rate of manual microscopy(12.39％,187/1509)was higher than that of OF-AS(11.73％,177/1509),but the difference was not statistically significant(x2=0.576,P＞0.05).Using MGIT 960 culture as the reference standard,the sensitivity and specificity of OF-AS for detecting tuberculosis were 51.76％(103/199)and 95.39％(290/304),respectively,which were slightly lower than those of manual microscopy(sensitivity:52.43％,108/206;specificity:96.97％,288/297).Both diagnostic methods showed moderate agreement with the gold standard(Kappa values:0.529 and 0.508,respectively).A total of 54 sputum smears had discordant results between OF-AS and manual microscopy,mainly due to missed detection by the scanner and interference from staining sediment.Eight sputum smears showed discordance between initial manual microscopy and repeated manual microscopy,all attributed to missed detection under microscopy.Conclusion:OF-AS(software recognition)has good application value in testing sputum specimens of suspected pulmonary tuberculosis patients,and its detection performance can meet the requirements of implementing traditional auramine O staining project for detecting mycobacterium.关键词
分枝杆菌,结核/显微镜检查,荧光/诊断,鉴别Key words
Mycobacterium tuberculosis/Microscopy,fluorescence/Diagnosis,differential分类
医药卫生引用本文复制引用
江丽娜,戴文汐,巨韩芳,孟苏凯,王春花..金胺O荧光染色全自动显微扫描识别系统在结核病诊断中的应用价值[J].中国防痨杂志,2026,48(4):504-509,6.基金项目
天津卫生健康行业高层次人才选拔培养工程项目(TJSJMYXYC-D2-017) Tianjin Health Industry High-level Talents Selection and Training Project(TJSJMYXYC-D2-017) （TJSJMYXYC-D2-017）
