中国中医急症2026，Vol.35Issue(3)：265-269,278,6.DOI:10.3969/j.issn.1004-745X.2026.03.004
栀子提取液对急性出血坏死性胰腺炎小鼠炎性介质的影响研究
Effects of Gardenia Extract on Inflammatory Mediators in Mice with Acute Hemorrhagic Necrotizing Pancreatitis
摘要
Abstract
Objective:To investigate the effects of gardenia extract on inflammatory mediators in mice with acute hemorrhagic necrotizing pancreatitis.Methods:BALB/c mice were randomly divided into the sham opera-tion group,model group,gardenia group and activator group,with 8 mice in each group.The acute hemorrhagic necrotizing pancreatitis model was established,and each group was administered the corresponding drug treatment.The serum levels of IL-6,IL-1β,TNF-α,IL-4,IL-10,amylase and lipase were detected;the pancreatic index was calculated;hematoxylin-eosin(HE)staining was used to observe the pathological changes of pancreatic tissue and score the damage;immunohistochemical staining was applied to detect the expression of myeloperoxidase(MPO)in pancreatic tissue;Western blotting was performed to determine the protein expressions of p38MAPK,p-p38MAPK,NF-κB p65 and p-NF-κB p65 in pancreatic tissue.Results:Compared with the sham operation group,the serum levels of pro-inflammatory factors(TNF-α,IL-1β,IL-6),amylase,lipase,pancreatic index,number of MPO-posi-tive cells,and protein expressions of p-p38MAPK and p-NF-κB p65 in pancreatic tissue of the model group were significantly increased,while the serum levels of anti-inflammatory factors(IL-4,IL-10)were significantly de-creased(P<0.001).Compared with the model group,the serum levels of pro-inflammatory factors(TNF-α,IL-1β,IL-6),amylase,lipase,pancreatic index,number of MPO-positive cells,and protein expressions of p-p38MAPK and p-NF-κB p65 in pancreatic tissue of the gardenia group were significantly decreased,while the serum levels of anti-inflammatory factors(IL-4,IL-10)were significantly increased(P<0.001).Compared with the gardenia group,the serum levels of pro-inflammatory factors(TNF-α,IL-1β,IL-6),amylase,lipase,pancreatic index,num-ber of MPO-positive cells,and protein expressions of p-p38MAPK and p-NF-κB p65 in pancreatic tissue of the activator group were significantly increased,while the serum levels of anti-inflammatory factors(IL-4,IL-10)were significantly decreased(P<0.001).Conclusion:Gardenia extract can inhibit the inflammatory response and improve the pathological damage of pancreatic tissue in mice with acute hemorrhagic necrotizing pancreatitis,and its mechanism may be related to the inhibition of p38MAPK/NF-κB signaling pathway activation.关键词
急性出血坏死性胰腺炎/栀子提取液/p38MAPK/NF-κB信号/炎症反应/小鼠Key words
Acute hemorrhagic necrotizing/Gardenia extract/p38MAPK/NF-κB signaling pathway/Inflammato-ry response/Mice分类
医药卫生引用本文复制引用
赵芳,陈淼,陈才利..栀子提取液对急性出血坏死性胰腺炎小鼠炎性介质的影响研究[J].中国中医急症,2026,35(3):265-269,278,6.基金项目
海南省高等学校科学研究项目(Hnky2024ZD-10) （Hnky2024ZD-10）
