中国蔬菜Issue(3)：70-80,11.DOI:10.19928/j.cnki.1000-6346.2025.5058
黄瓜韧皮部核心细菌群落结构及其功能解析
Study of Core Bacterial Community Composition and Function in Phloem Sap of Cucumber
摘要
Abstract
Plant endophytic microorganisms play a crucial role in regulating host nutrient absorption and stress resistance.However,the mechanisms by which host genetic factors drive the formation of specific microbial communities remain unclear.This study investigated cucumber,a model crop with a vascular system,focusing on the phloem-a key site for assimilate transport.Using 16S rRNA amplicon sequencing,we systematically analyzed the composition characteristics of endophytic bacterial communities in phloem tissues across different cucumber genotypes.Results showed that Proteobacteria dominated the phloem bacterial communities in all genotypes.Thirteen core amplicon sequence variants(ASVs)were identified,with culturable strains successfully isolated.Genome-wide association study(GWAS)revealed that the relative abundance of core ASV Stenotrophomonas_ASV1 showed significant association with SNP locus Chr4_24 974 285.The corresponding gene CsaV3_4G035400,encoding a xylanase-active protein,was identified as a potential key regulator of microbial recruitment.Functional validation demonstrated that this bacterial strain significantly promotes cucumber seedling growth.Our findings highlight the potential role of plant xylanase-related genes in regulating specific endophyte recruitment,providing new insights into host gene-microbe interaction mechanisms.关键词
黄瓜/韧皮部细菌群落结构/16SrRNA扩增子测序/全基因组关联分析Key words
cucumber/phloem bacterial community structure/16S rRNA amplicon sequencing/genome-wide association study(GWAS)引用本文复制引用
朱雪莹,王昆,郑盈盈,叶晓龙,柴森,魏海雷,秦宇轩,杨学勇..黄瓜韧皮部核心细菌群落结构及其功能解析[J].中国蔬菜,2026,(3):70-80,11.基金项目
中国农业科学院创新工程项目(CAAS-ASTIP-2025-IVF) （CAAS-ASTIP-2025-IVF）
