浙江农林大学学报2026，Vol.43Issue(2)：303-309,7.DOI:10.11833/j.issn.2095-0756.20250186
过表达紫花苜蓿ChOMT基因创制高异黄酮营养强化番茄
Overexpression of Medicago sativa ChOMT gene in constructing homoisoflavone-enriched tomatoes
摘要
Abstract
[Objective]This study aims to construct transgenic homoisoflavones tomato(Solanum lycopersicum)plants based on the homoisoflavones synthase gene ChOMT from alfalfa(Medicago sativa),thereby achieving nutritional fortification of tomatoes.[Method]ChOMT gene was introduced into tomatoes using agrobacterium-mediated transformation.Transgenic identification was carried out through PCR and fluorescence detection.Furthermore,plant-wide targeted metabolomics technology and multivariate statistical analysis methods were employed for precise quantitative analysis of homoisoflavones content.[Result]Through PCR and fluorescence detection,positive transgenic plants of ChOMT were obtained.Compared with the wild type,the content of homoisoflavonoids significantly increased.Among them,the relative content of(3RS)-5,7-dihydroxy-3-(2-hydroxy-4-methoxybenzyl)-chroman-4-one(HIF1)in the leaves of transgenic tomatoes was 57 019,which was 260 times higher than the relative content of 220 in the leaves of the wild type.T he relative content of 5,7-dihydroxy-3-(4-hydroxybenzyl)-chroman-4-one(HIF2)in the fruits of transgenic tomatoes was 15 849 985,which was over 4 000 times higher than the relative content of 3 849 in the wild type.[Conclusion]ChOMT transgenic tomatoes are obtained,which not only provides a theoretical basis for analyzing the catalytic function of ChOMT in plants,but also lays an important foundation of germplasm resources for breeding genetically improved tomato varieties with high homoisoflavonoid content.[Ch,6 fig.28 ref.]关键词
高异黄酮/番茄/农杆菌介导法/ChOMT/代谢组/分子农场Key words
homoisoflavones/tomato/agrobacterium-mediated method/ChOMT/metabolome/molecular farm分类
农业科技引用本文复制引用
张杨,诸燕,韩之刚,杨美森,斯金平,陈东红..过表达紫花苜蓿ChOMT基因创制高异黄酮营养强化番茄[J].浙江农林大学学报,2026,43(2):303-309,7.基金项目
浙江省重点研发专项(2022C02009) （2022C02009）
