中国饲料Issue(7)：65-71,7.DOI:10.15906/j.cnki.cn11-2975/s.2024110049-01
菌酶协同发酵饲料的生产工艺及体外消化研究
A study of production process and of digestion in vitro of fermented feed by synergistic fermentation of multi-microbial and enzyme
摘要
Abstract
The fermented feed was produced using corn gluten meal and soybean meal and wheat bran as the main raw materials by synergistic fermentation with multi-microbial and enzyme.The production process were optimized by single factor experiment with the soluble protein content as indicators,and the digestibility the digestion of fermented feed were evaluated by in vitro simulation digestion method.The results showed as follows:mass ratio of corn gluten meal,soybean meal,wheat bran,corn steep liquor,molasses in medium was 7∶7∶6∶0.1∶0.2,the moisture content was 45％(V/m)in the medium.The complex strains were composed of Bacillus subtilis,Saccharomyces cerevisiae and Lactobacillus acidophilus,the ratio of seed liquid was 1∶1∶1(V/V/V),and inoculation amount was 5％(V/m),the addition of neutral protease was 100 U/g.fermentation temperature was 35 ℃,and fermentation time was 4 days.Under the optimized fermentation conditions,the content of soluble protein was 199.05 mg/g,the content of polypeptide was 102.24 mg/g,and the number of living microbial cells was 3.67×107 CFU/g.Compared with unfermented feed,the digestibility of dry matter in fermented feed was increased by 13.25 percentage points(P＜0.05),and digestibility of crude protein was increased by 13.01 percentage points(P＜0.05)after simulated stomach and intestinal digestion in vitro,and the digestibility of dry matter in fermented feed was increased by 6.16 percentage points(P＜0.05),and digestibility of crude protein was increased by 7.67 percentage points(P＜0.05)after simulated intestinal digestion in vitro.In conclusion,the nutrient value and digestibility of feed were significantly improved by synergistic fermentation with multi-microbial and enzyme,which had wide application prospects in biological feed industries.关键词
菌酶协同发酵/发酵饲料/可溶性蛋白/优化/体外模拟消化Key words
synergistic fermentation with multi-microbial and enzyme/fermented feed/soluble protein/optimization/in vitro simulated digestion分类
农业科技引用本文复制引用
邓永平,李天龙,李冠龙,刘晓兰,李闯..菌酶协同发酵饲料的生产工艺及体外消化研究[J].中国饲料,2026,(7):65-71,7.基金项目
黑龙江省植物性食品加工技术优势特色学科"科技攻关"专项(YSTSXK202203) （YSTSXK202203）
黑龙江省教育厅基本科研业务专项(145309329) （145309329）
黑龙江省"百千万"工程科技重大专项(2020ZX06B01) （2020ZX06B01）
黑龙江省玉米深加工理论与技术重点实验室开放课题项目(SPKF202010) （SPKF202010）
