中国蔬菜Issue(3)：81-89,9.DOI:10.19928/j.cnki.1000-6346.2025.5034
SlProT1基因克隆、生物信息学分析及亚细胞定位
Cloning,Bioinformatics Analysis,and Subcellular Localization Study of SlProT1
摘要
Abstract
ProT is a type of amino acid transporter located on the plasma membrane,belonging to the AAAP family,responsible for the transport of amino acids from the apoplast to the cytosol.This experiment cloned and performed bioinformatics analysis and localization analysis on the protein encoded by SlProT1.The results showed that the SlProT1 encodes 441 amino acids,with a molecular weight of 48.54 Kda and a theoretical isoelectric point of 9.34.It is a stable hydrophobic protein with a protein structure including alpha helices,random coils,and extended strands.Real time fluorescence quantitative analysis showed that the SlProT1 is expressed in the root,stem,leaf,and flower tissues of tomatoes,with the highest expression level in the leaves,significantly higher than in the roots,stems,and flowers.Bioinformatics prediction shows that SlProT1 may be located on the plasma membrane and vacuolar membrane,while subcellular localization of SlProT1-mCherry comfirmed that SlProT1 is located on the plasma membrane.关键词
SlProT1/克隆/生信分析/亚细胞定位Key words
SlProT1/clone/bioinformatics analysis/subcellular localization引用本文复制引用
王学瑾,梁浩,李昊玥,王宝驹,季延海,胡丽萍..SlProT1基因克隆、生物信息学分析及亚细胞定位[J].中国蔬菜,2026,(3):81-89,9.基金项目
北京市农林科学院青年基金项目(QNJJ202328),北京市乡村振兴农业科技项目(NY2401080000) （QNJJ202328）
