浙江农林大学学报2026，Vol.43Issue(2)：331-339,9.DOI:10.11833/j.issn.2095-0756.20250221
3种黄连木属植物叶绿体基因组结构特征及遗传关系
Structural characteristics and genetic relationship of chloroplast genomes of 3 Pistacia species
摘要
Abstract
[Objective]In this study,the chloroplast genomes of 3 Pistacia species were compared to analyze their structural characteristics and genetic evolution.[Method]3 publicly available chloroplast genomes of Pistacia species from National Center for Biotechnology Information(NCBI)were selected as research materials,and their chloroplast genome structure,repeat sequence,nucleotide polymorphism and genetic relationship were analyzed by relevant bioinformatics methods.[Result]The chloroplast genomes of the 3 species were tetrad ring structure,and the numbers of protein-coding sequence(CDS),ribosomal RNA(rRNA),and transfer RNA(tRNA)were exactly the same among them;The simple repeat sequences were mainly A(34.35%)or T(39.69%)repeat units in mono-nucleotide,but no hexa-nucleotide.And the number of scattered r epeat sequences were mainly palindromic repeats(105)and forward repeats(76),but no reverse repeats;There were different degrees of variation in the conserved non-coding sequences(CNS)of the large single copy(LSC)and small single copy(SSC)of the chloroplast genomes of three species,but there was no significant difference in the contraction or expansion of genes near the tetrad boundary;5 highly variable sequences(matK,trnG-UCC～trnR-UCU,trnT-UGU～trnL-UAA,petD～rpoA,rpl22～rpl2)were identified in the LSC region and the boundary between LSC and inverted repeat b(IRb);Phylogenetic reconstruction based on complete chloroplast genomes demonstrated clear segregation between Pistacia and Rhus,and P.chinensis was closely related to P.weinmaniifolia,while P.vera and P.atlantica could be formed a distinct clade.[Conclusion]The chloroplast genome structure of the 3 Pistacia species was similar and relatively conservative,and the number of various genes was consistent;The 5 mutant sequences detected could be used as candidate molecular markers for Pistacia species;The genetic relationship between P.chinensis and P.weinmannifolia was similar,while P.vera was relatively distant from former 2 species.[Ch,6 fig.1 tab.46 ref.]关键词
黄连木属/叶绿体基因组/重复序列/遗传关系Key words
Pistacia/chloroplast genome/sequence repeat/genetic relationship分类
农业科技引用本文复制引用
周泽霖,向长武,张少军,陈日美,何杰祖,牙璋,黄毅,劳广杰..3种黄连木属植物叶绿体基因组结构特征及遗传关系[J].浙江农林大学学报,2026,43(2):331-339,9.基金项目
广西生态工程职业技术学院自然科学课题(2024KY08) （2024KY08）
广西林业科技项目(2024GXZCLK03) （2024GXZCLK03）
