中国医科大学学报2026，Vol.55Issue(3)：259-264,271,7.DOI:10.12007/j.issn.0258-4646.2026.03.011
异氟醚调节AMPK/mTOR信号通路减轻大鼠缺氧/复氧所致的心肌细胞损伤
Isoflurane alleviates hypoxia/reoxygenation-induced cardiomyocyte injury in rats via regulating AMPK/mTOR signaling pathway
摘要
Abstract
Objective To investigate the mechanisms through which isoflurane alleviates hypoxia/reoxygenation(H/R)injury to cardio-myocytes via regulating the AMPK/mTOR signaling pathway.Methods Using H/R-induced rat cardiomyocytes,H9c2 cells were treated with different isoflurane concentrations,and the optimal isoflurane concentration was determined using an MTT assay.H9c2 cells were sto-chastically assigned to control,H/R,isoflurane,compound C(CC),and isoflurane+CC groups.All groups other than the control group were subjected to H/R treatment.After treatment with isoflurane and the AMPK inhibitor CC,the cell viability,mitochondrial membrane poten-tial(∆Ψm),and apoptosis rate were detected in each group using the CCK-8 method,JC-1 fluorescence staining,and flow cytometry,respectively.Transmission electron microscopy and flow cytometry were used to observe the ultrastructure of the H9c2 cells and detect the levels of reactive oxygen species(ROS)in the cells in each group,respectively.The antioxidant enzyme activity and levels of inflammatory factors released in the H9c2 cells were measured in all groups.Western blotting was used to detect the expression levels of AMPK/mTOR pathway proteins in the H9c2 cells in each group.Results The cell viability,∆Ψm,catalase(CAT)and superoxide dismutase(SOD)activities,and p-AMPK/AMPK were lower in H/R group than in the control group,whereas the apoptosis rate;ROS,interleukin-6(IL-6),and tumor necrosis factor α(TNF-α)levels;and p-mTOR/mTOR were higher in the H/R group than in the control group(all P<0.05).The cell viability and ∆Ψm were higher in the isoflurane group than in the H/R group.The apoptosis rate;ROS,IL-6,and TNF-α levels;and p-mTOR/mTOR were lower the isoflurane group than in the H/R group.The changes in the various parameters in the cells in the CC group were the opposite to those in isoflurane group(all P<0.05).The cell viability and ∆Ψm were lower in the isoflurane+CC group compared with those in the isoflurane group,whereas the apoptosis rate;ROS,IL-6,and TNF-α levels;and p-mTOR/mTOR were higher in the isoflurane+CC group(all P<0.05).Conclusion Isoflurane alleviates H/R-induced cardiomyocyte injury via activating the AMPK/mTOR signaling pathway.关键词
异氟醚/AMPK/mTOR/缺氧/复氧/心肌细胞/保护作用Key words
isoflurane/AMPK/mTOR/hypoxia/reoxygenation/cardiomyocytes/protective function分类
医药卫生引用本文复制引用
陈艳林,张晓敏,刘娜,袁慧敏,邢珍,姚杰..异氟醚调节AMPK/mTOR信号通路减轻大鼠缺氧/复氧所致的心肌细胞损伤[J].中国医科大学学报,2026,55(3):259-264,271,7.基金项目
河北省医学科学研究课题(20241120) （20241120）
