浙江农林大学学报2026，Vol.43Issue(2)：381-394,14.DOI:10.11833/j.issn.2095-0756.20250227
三江源退化高寒草甸土壤代谢产物特征及其与土壤环境因子的相关性
Characteristics of soil metabolites associated with degraded alpine meadow and their correlations with environmental factors in Sanjiangyuan region
摘要
Abstract
[Objective]This study examines soil metabolite variations in degraded alpine meadows of the Sanjiangyuan region,identifies key metabolic pathway shifts,and assesses their correlations with soil environmental factors.The findings provide scientific insights into degradation drivers and soil ecological responses.[Method]This study focuses on alpine meadows in Sanjiangyuan National Park,Maqin County,Guoluo Tibetan Autonomous Prefecture,Qinghai Province.It uses untargeted LC-MS/MS metabolomics to analyze soil metabolites across a degradation gradient,including non-degraded,lightly degraded,moderately degraded,and severely degraded meadows.The study also examines correlations between key differentially expressed metabolites and soil environmental factors.[Result]A total of 8 451 metabolites were identified,with lipids and lipid-like molecules being the most abundant(26.81%).Soil degradation significantly altered metabolite types and contents,with the most significant differences observed between heavily degraded and other meadow types.The top 20 signature metabolites included carboxylic acids,fatty acyls,organic nitrogen compounds,and sphingolipids.Key metabolic pathways affected were linoleic acid metabolism,ABC transporters,phospholipase D signaling,and sphingolipid signaling.Redundancy analysis revealed significant correlations between soil metabolites and environmental factors such as organic matter,water content,total nitrogen,total phosphorus,pH,and urease activity.[Conclusion]The types of signature metabolites,differentially expressed metabolites,and major metabolic pathways in alpine meadow soils varied significantly across degradation levels.These variations were hypothesized to correlate strongly with changes in environmental factors such as soil organic matter,water content,total nitrogen,total phosphorus,pH,and soil urease.[Ch,6 fig.2 tab.55 ref.]关键词
高寒草甸/退化/非靶向代谢组学/差异代谢物/土壤理化性质和酶活性Key words
alpine meadows/degradation/non-targeted metabolomics/differential metabolites/soil physicochemical properties and enzyme activity分类
农业科技引用本文复制引用
杨晓燕,金辉,戴璐,黄先成,陈吉祥,秦波..三江源退化高寒草甸土壤代谢产物特征及其与土壤环境因子的相关性[J].浙江农林大学学报,2026,43(2):381-394,14.基金项目
中央引导地方科技发展资金项目(24ZYQA043) （24ZYQA043）
中国科学院战略性先导科技专项(A类)(XDA26020201) （A类）
甘肃省知识产权计划资助项目(24ZSCQG013) （24ZSCQG013）
甘肃省科技计划资助项目(23JRRA604,24JRRA065) （23JRRA604,24JRRA065）
