中国中医药信息杂志2026，Vol.33Issue(4)：41-49,9.DOI:10.19879/j.cnki.1005-5304.202509638
基于网络药理学和实验探究清肝降浊方调控氧化应激及炎症治疗代谢相关脂肪性肝病机制
Exploration on the Mechanism of Qinggan Jiangzhuo Prescription in Regulating Oxidative Stress and Inflammation for Metabolic Related Fatty Liver Disease Based on Network Pharmacology and Experimental Research
摘要
Abstract
Objective To investigate the mechanism of Qinggan Jiangzhuo Prescription in the treatment of metabolic associated fatty liver disease(MAFLD)rats.Methods The active component targets of Qinggan Jiangzhuo Prescription and the disease targets of MAFLD were screened by using the network pharmacology related database.The protein-protein interaction network was constructed by taking the intersection targets of the two,and the GO enrichment analysis and KEGG pathway enrichment analysis were carried out.High-fat methionine restricted choline deficient diet was used to establish MAFLD rat model.The rats were divided into blank group,model group,silibinin group,Qinggan Jiangzhuo Prescription equivalent dosage group,Qinggan Jiangzhuo Prescription high-dosage group,and given corresponding intervention for 8 weeks.Serum alanine aminotransferase,aspartate aminotransferase,total cholesterol,triglyceride,high density lipoprotein cholesterol and low density lipoprotein cholesterol were detected in each group.HE staining and NAFLD activity score of nonalcoholic fatty liver disease were used to evaluate the pathological changes of liver tissue.The TNF-α,IL-1β,IL-6,MDA,T-SOD,CAT and GSH in liver tissue were detected by ELISA.The expressions of oxidative stress inflammation related proteins in liver tissue were detected by Western blot.Results Network pharmacology results showed that the core components of Qinggan Jiangzhuo Prescription included quercetin,kaempferol,luteolin,etc.,which may exert a protective effect on MAFLD rats by regulating the Akt/Nrf2/NFκB axis and its upstream and downstream targets related to the oxidative stress inflammation pathway.Animal experiment results showed that Qinggan Jiangzhuo Prescription could reduce serum transaminase levels,improve serum lipid mass spectrometry expression,lower TNF-α,IL-1β,IL-6,MDA and increase T-SOD,CAT and GSH in liver tissue of MAFLD rats,down-regulate the expressions of Akt,IKKβ,NF-κB and Keap1 in liver tissue,and up-regulated the expressions of IκBα,Nrf2 and HO-1,with statistical significance(P<0.05,P<0.01).Conclusion Qinggan Jiangzhuo Prescription has the characteristics of multi-target and multi pathway in the treatment of MAFLD.It may alleviate oxidative stress and inflammatory response by regulating the Akt/Nrf2/NFκB signaling axis,thereby exerting therapeutic effects on MAFLD.关键词
清肝降浊方/代谢相关脂肪性肝病/氧化应激/炎症/Akt/Nrf2/NFκB信号通路/网络药理学Key words
Qinggan Jiangzhuo Prescription/metabolic related fatty liver disease/oxidative stress/inflammation/Akt/Nrf2/NFκB signaling pathway/network pharmacology分类
医药卫生引用本文复制引用
周天惠,廖镜林,蒋婷,宋依蓬,许卫华,姚树坤..基于网络药理学和实验探究清肝降浊方调控氧化应激及炎症治疗代谢相关脂肪性肝病机制[J].中国中医药信息杂志,2026,33(4):41-49,9.基金项目
中日友好医院生物医学转化工程系列研究项目(PYBZ1815) （PYBZ1815）
北京市科技计划-G20工程创新研究(Z171100001717008) （Z171100001717008）
北京中医药大学教育科学研究课题(XBB24037) （XBB24037）
