中国现代中药2026，Vol.28Issue(3)：584-591,8.DOI:10.13313/j.issn.1673-4890.20250620003
基于HPLC特征图谱和一测多评法的经典名方桔梗汤质量控制研究
Quality Control of the Classic Formula Jiegeng Decoction Based on HPLC Characteristic Chromatograms and Quantitative Analysis of Multi-components by Single Marker Method
摘要
Abstract
Objective:To establish the high-performance liquid chromatography(HPLC)characteristic chromatograms of the classic formula Jiegeng Decoction and determine the content of its major components using the quantitative analysis of multi-components by single marker(QAMS)method.Methods:Separation was conducted on gradient elution through a Phenomenex-C18 column(250 mm×4.6 mm,5 μm)with a mobile phase consisting of 0.05%phosphoric acid aqueous solution(A)and acetonitrile(B)at a column temperature of 35℃and a flow rate of 1.0 mL·min-1.Detection was performed with a diode array detector at a wavelength of 203 nm.The HPLC characteristic chromatograms of Jiegeng Decoction were established,and the common peaks were identified and assigned.The similarity between the HPLC characteristic chromatograms of 15 batches of Jiegeng Decoction and the reference characteristic chromatogram was evaluated.The QAMS method was employed to determine the content of four major components(glycyrrhizin,glycyrrhetic acid,deapioplatycodin E,and platycodin D)in Jiegeng Decoction.Results:The HPLC characteristic chromatograms of Jiegeng Decoction were successfully established,with 14 characteristic peaks identified.The peaks 5,9,12,and 14 were identified as glycyrrhizin,glycyrrhetic acid,deapioplatycodin E,and platycodin D,respectively.Glycyrrhizin and glycyrrhetic acid were derived from Glycyrrhizae Radix et Rhizoma,while deapioplatycodin E and platycodin D were from Platycodonis Radix.Furthermore,the similarity between the characteristic chromatograms of the fifteen batches of Jiegeng Decoction and the reference characteristic chromatogram was greater than 0.900.The contents of deapioplatycodin E,platycodin D,glycyrrhizin,and glycyrrhetic acid in the 15 batches of Jiegeng Decoction were in the ranges of 0.31-0.35,5.43-6.14,6.31-7.05,and 10.35-12.45 mg·g-1,respectively.Conclusion:The established HPLC characteristic chromatograms combined with the QAMS method are efficient and feasible,providing a comprehensive scientific basis for the quality evaluation of the classic formula Jiegeng Decoction.关键词
桔梗汤/特征图谱/一测多评法/含量测定/相似度Key words
Jiegeng Decoction/characteristic chromatograms/quantitative analysis of multi-components by single marker(QAMS)/content determination/similarity分类
医药卫生引用本文复制引用
吴成志,熊梦琪,乔金为,徐倩,葛丹丹,汪宏雷,蔡明..基于HPLC特征图谱和一测多评法的经典名方桔梗汤质量控制研究[J].中国现代中药,2026,28(3):584-591,8.基金项目
安徽省2024年优秀青年教师培育项目(YQYB2024030) （YQYB2024030）
安徽中医药大学第二附属医院人才支持计划(杏林计划)项目(院发[2023]118号) （杏林计划）
