中国肿瘤生物治疗杂志2026，Vol.33Issue(2)：174-180,7.DOI:10.3872/j.issn.1007-385x.2026.02.009
SHCBP1通过调控PI3K/AKT/mTOR信号通路促进结直肠癌Caco-2细胞增殖、迁移和侵袭
SHCBP1 promotes proliferation,migration,and invasion of colorectal cancer Caco-2 cells by regulating the PI3K/AKT/mTOR signaling pathway
摘要
Abstract
Objective:To investigate the expression of Shc SH2 domain-binding protein 1(SHCBP1)in colorectal cancer(CRC)and its regulatory mechanism on Caco-2 cell proliferation,migration,and invasion.Methods:Caco-2 cells were divided into four experimental groups:the Con group(blank control),the sh-NC group(transfection with negative control sh-NC),the sh-SHCBP1 group(transfection with sh-SHCBP1),and the sh-SHCBP1+740Y-P group(transfection with sh-SHCBP1 followed by treatment with the PI3K activator 740Y-P at 30 μmol/L for 1 h).Cell proliferation,migration,and invasion were assessed using the CCK-8 assay,wound-healing,and Transwell invasion assay,respectively.Vasculogenic mimicry(VM)formation in Caco-2 cells was examined using a Matrigel-based three-dimensional culture system.The mRNA expression level of SHCBP1 was detected using qRT-PCR,and the protein expression levels of SHCBP1,VM-related markers,and PI3K/AKT/mTOR signaling pathway-related proteins were detected using WB.Results:SHCBP1 was significantly upregulated in CRC tissues and cell lines(P＜0.05).Compared with Con group and sh-NC group,the sh-SHCBP1 group showed significantly reduced mRNA and protein levels of SHCBP1,cell proliferation activity,wound-healing rate,number of invaded cells,and number of formed tubules(all P＜0.05).In addition,the protein expression levels of hypoxia-inducible factor 1α(HIF-1α),ephrin type-A receptor 2(EPHA2),VEGFA,p-PI3K,p-AKT,and phosphorylated mTOR(p-mTOR)were significantly decreased(all P＜0.05).Compared with the sh-SHCBP1 group,the sh-SHCBP1+740Y-P group demonstrated significantly increased cell proliferation,wound-healing rate,number of invaded cells,and number of formed VM structures(all P＜0.05),along with significantly elevated protein expression of HIF-1α,EPHA2,VEGFA,p-PI3K,p-AKT,and p-mTOR(all P＜0.05).Conclusion:SHCBP1 is highly expressed in CRC cells and promotes the proliferation,migration,and invasion of Caco-2 cells.The underlying mechanism may be associated with activation of the PI3K/AKT/mTOR signaling pathway and subsequent promotion of the VM process.关键词
SHCBP 1/PI3K/AKT/mTOR/结直肠癌/血管生成拟态/Caco-2细胞Key words
SHCBP1/PI3K/AKT/mTOR/colorectal cancer(CRC)/vasculogenic mimicry(VM)/Caco-2 cell分类
医药卫生引用本文复制引用
李丽,姚红亮..SHCBP1通过调控PI3K/AKT/mTOR信号通路促进结直肠癌Caco-2细胞增殖、迁移和侵袭[J].中国肿瘤生物治疗杂志,2026,33(2):174-180,7.基金项目
河北省医学科学研究课题计划(20211322) （20211322）
