中国现代中药2026，Vol.28Issue(3)：605-613,9.DOI:10.13313/j.issn.1673-4890.20250603003
黄芪肉桂饮中化学成分鉴定及糖化酶活力测定
Identification of Chemical Components and Determination of Glucosidase Activity of Huangqi Rougui Decoction
摘要
Abstract
Objective:To establish an ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q/TOF-/MS)method for the rapid characterization of chemical components in Huangqi Rougui Decoction and measure its glucosidase activity using the high performance liquid chromatography-evaporative light scattering detector(HPLC-ELSD)method.Methods:A Waters Acquity UPLC BEH C18 column(100 mm×2.1 mm,1.7 μm)was used for gradient elution with acetonitrile and 0.1%formic acid aqueous solution.MS data were collected through the utilization of electrospray ionization(ESI)in both positive and negative ion modes.The chemical component databases of Astragali Radix and Cinnamomi Cortex were established based on literature review.The UNIFI platform was used to compare the molecular weights and MS² fragment ion information of the chemical components in Huangqi Rougui Decoction with the established database for component identification,and the possible monosaccharides produced after fermentation were analyzed.The glucosidase activity in Huangqi Rougui Decoction was determined via a Prevail Carbohydrate ES column(250 mm×4.6 mm,5 μm)with the mobile phase consisting of acetonitrile and water at a ratio of 75:25,the column temperature of 30℃,and the flow rate of 1.0 mL·min-1.For the ELSD detector,the drift tube temperature was 115℃and the gas flow rate was 3 L·min-1.Results:A total of 17 compounds were identified in Huangqi Rougui Decoction.The HPLC-ELSD method demonstrated good linearity for D-glucose within the concentration range of 114.9-1 148.9 μg·mL-1(r=0.999 1).The limit of detection and limit of quantification were 30 μg·mL-1 and 50 μg·mL-1,respectively.The glucosidase activities of the five batches of Huangqi Rougui Decoction samples were 27.97,29.53,29.20,31.32,and 29.05 U·μmol-1,respectively.Conclusion:UPLC-Q/TOF-MS combined with the UNIFI platform allowed the rapid identification of the chemical components in Huangqi Rougui Decoction and the HPLC-ELSD method was proved effective for the measurement of glucosidase activity in Huangqi Rougui Decoction.关键词
糖化酶活力/黄芪肉桂饮/蒸发光散射检测器法Key words
glucosidase activity/Huangqi Rougui Decoction/evaporative light scattering detector分类
医药卫生引用本文复制引用
武哲,武浩楠,李明华,邱淦清,程显隆,魏锋..黄芪肉桂饮中化学成分鉴定及糖化酶活力测定[J].中国现代中药,2026,28(3):605-613,9.基金项目
国家重点研发计划项目(2023YFC3504105) （2023YFC3504105）
