浙江农业科学2026，Vol.67Issue(3)：591-594,4.DOI:10.16178/j.issn.0528-9017.20240513
甬马1号马铃薯外植体的再生体系优化
Optimization of regeneration system for explant from potato Yongma 1
摘要
Abstract
The establishment of regeneration system for potato explant played a crucial role in the fundamental theoretical research of potato breeding,detoxification and rapid propagation,as well as the metabolic,physiological,and biochemical aspects of cells and tissues.This experiment selected the variety Yongma 1 as the material,and applied different disinfection methods to treat buds of different lengths,so as to select the most suitable bud length and disinfection time for establishing regeneration system for potato explants,laying the foundation for forming a complete high-quality breeding system with tissue culture rapid propagation,greenhouse cutting,net room cultivation,original seed breeding,and qualified production as the chain.The results showed that the optimal apical bud length for the survival and growth of the potato explants of Yongma 1 was 1.0 to 1.5 cm.Apical buds that were too large or too small were not conducive to the establishment of regeneration system for potato explant.At the same time,the explants with apical buds washed in running water for 30-35 minutes,disinfected with 75%ethanol for 30 to 50 seconds,and disinfected with 0.1%HgCl2 for 180 to 300 seconds had the highest survival rate and the lowest contamination rate and browning rate.In addition,the optimal light intensity for the growth of potato explants was 3 000 to 5 000 lx,and the light duration was 12 to 18 hours.关键词
马铃薯/外植体/组织培养/消毒Key words
potato/explant/tissue culture/disinfection分类
农业科技引用本文复制引用
王芳,张宇旭,朱诗君,陈铁锋,金树权,严成其..甬马1号马铃薯外植体的再生体系优化[J].浙江农业科学,2026,67(3):591-594,4.基金项目
宁波市公益性研究计划项目(2024S014) （2024S014）
浙江省农业新品种选育重大科技专项子课题(2021C02064-1-5) （2021C02064-1-5）
慈溪市科技局重大公益项目(CZ2022003) （CZ2022003）
