中山大学学报（自然科学版）（中英文）2026，Vol.65Issue(2)：99-108,10.DOI:10.13471/j.cnki.acta.snus.ZR20250170
高温胁迫和恢复对牛蛙蝌蚪及幼蛙生长、变态和抗氧化功能的影响
Effects of high temperature stress and recovery on growth,metamorphosis and antioxidant function of bullfrog(Lithobates catesbeiana)in tadpoles and juvenile stage
摘要
Abstract
To investigate the physiological regulation of bullfrogs(Lithobates catesbeiana)under long-term heat stress,this study simulated a high-temperature and recovery environment to examine the effects of water temperature on growth,metamorphosis,and antioxidant function during the tadpole and juvenile stages.Three thousand six hundred bullfrog tadpoles at Gosner stage 25[7 d old;(0.04±0.00)g]were randomly divided into 12 tanks,with 300 individuals per tank,and reared for 80 days at water temperatures of 24,27,and 30℃,respectively.Subsequently,post-metamorphic juveniles were maintained at 24℃for an additional 56 days.The results showed that,during the tadpole stage,final body weight(FBW),weight gain rate(WGR),and specific growth rate(SGR)did not differ significantly between the 24 and 27℃groups(P＞0.05),but their values were all significantly higher than those of tadpoles in the 30℃group(P＜0.05).The male ratio and metamorphosis rate in the 27℃group were significantly higher than those in the 24℃group,but lower in the 30 ℃ group(P＜0.05).For juveniles reared at 24℃,FBW,WGR,SGR,and survival rate showed no significant differences between the 24 and 27℃groups(P＞0.05),but their values were all significantly higher than those in the 30℃group(P＜0.05).In terms of intestinal enzyme activity,protease and creatine kinase activities in juveniles did not differ significantly between the 24 and 27℃groups(P＞0.05),but both were significantly lower than those in the 30 group(P＜0.05);lipase and acid phosphatase activities in the 24℃group were significantly higher than those in the 27 and 30℃groups(P＜0.05).Regarding antioxidant indices,intestinal superoxide dismutase(SOD)and catalase(CAT)activities in the 24 and 27℃groups were not significantly different(P＞0.05),but both were significantly higher than those in the 30℃group(P＜0.05).In the liver,glutamic pyruvic transaminase,glutamic oxaloacetic transaminase,SOD,and CAT activities,as well as malondialdehyde content,did not differ significantly between the 24 and 27℃groups(P＞0.05),but all were significantly lower than those in the 30℃group(P＜0.05).The hepatic total antioxidant capacity values of juveniles in the 27 and 30℃groups were significantly higher than those in the 24℃group(P＜0.05).In conclusion,a water temperature of 27℃can promote the growth performance,metamorphosis rate,and male ratio of bullfrog tadpoles,and can enhance the antioxidant capacity of juveniles after stress,thereby improving juvenile growth performance.These findings suggest that maintaining 27℃during the tadpole stage could optimize bullfrog aquaculture in subtropical regions under summer heat waves.关键词
高温胁迫/牛蛙/生长/变态/抗氧化能力/氧化应激Key words
high temperature stress/bullfrogs/growth/metamorphosis/antioxidant capacity/oxidative stress分类
农业科技引用本文复制引用
丁昊,何颖琳,李沃杏,潘燕芳,陈卓,徐超,杨慧荣..高温胁迫和恢复对牛蛙蝌蚪及幼蛙生长、变态和抗氧化功能的影响[J].中山大学学报（自然科学版）（中英文）,2026,65(2):99-108,10.基金项目
广东省科技厅"百千万工程"农村科技特派员第二轮重点派驻项目(KTP20240658) （KTP20240658）
农业农村部农产品质量安全风险评估项目(20244088) （20244088）
农业农村部农产品质量安全风险评估项目(20254059) （20254059）
