遵义医科大学学报2026，Vol.49Issue(3)：252-259,8.
新兴ST950型肺炎克雷伯菌的耐药性、毒力及基因组特征研究
Antimicrobial resistance,virulence,and genomic characteristics in the emer-ging klebsiella pneumoniae clone
摘要
Abstract
Objective To characterize the antimicrobial resistance,virulence traits,biological characteristics,and population structure of the ST950 KP clone.Methods A representative ST950 strain,KP230524-43,isola-ted from the urine of a patient with a UTI,was identified using the VITEK-2 Compact automated microbiology system.Its cytotoxicity and biofilm formation capacity were assessed via lactate dehydrogenase(LDH)release assay and crystal violet staining,respectively.The genomic DNA of klebsiella pneumoniae KP230524-43 was ex-tracted and subjected to whole-genome sequencing analysis.Phylogenetic tree analysis was performed using a to-tal of 23 klebsiella pneumoniae strains of sequence type 950(ST950),including 22 known sequences obtained from the NCBI database.Results KP230524-43 exhibited significant cytotoxicity,strong biofilm formation,and resistance to β-lactam antibiotics.Unlike classical hypervirulent strains,all ST950 isolates lacked common hy-pervirulence markers(e.g.,rmpA,peg-344,iucA).However,they consistently carried the enterobactin syn-thesis and transport genes entAB and fepC,the pilus synthesis gene cluster ecpABCDR,and the gene encoding outer membrane protein A(ompA).The astA gene,encoding the EAST1 toxin,was detected in some ST950 strains,including KP230524-43.Over half of the strains harbored carbapenemase or extended-spectrum β-lacta-mase resistance genes.Furthermore,our analysis revealed that ST950 strains have emerged in multiple coun-tries,indicating a potential for cross-border transmission.Conclusion The ST950 clone represents an emerging,potentially high-risk lineage of K.pneumoniae characterized by a distinct virulence profile,robust biofilm-forming capacity,and multidrug resistance.Enhanced surveillance,molecular epidemiological studies,and targeted in-fection control measures are urgently needed.关键词
肺炎克雷伯菌/基因组/ST950/毒力/生物膜形成/抗生素耐药Key words
klebsiella pneumoniae/genome/ST950/virulence/biofilm formation/antimicrobial resistance分类
医药卫生引用本文复制引用
毛盼,刘凌云,蒋蓉,向德标,李昕..新兴ST950型肺炎克雷伯菌的耐药性、毒力及基因组特征研究[J].遵义医科大学学报,2026,49(3):252-259,8.基金项目
抗耐药微生物药物湖南省重点实验室(NO:2023TP1013) （NO:2023TP1013）
长沙市卫生健康科研课题(NO:KJ-202545) （NO:KJ-202545）
湖南中医药大学校院联合基金资助项目(NO:2025XYLH271). （NO:2025XYLH271）
