浙江农业科学2026，Vol.67Issue(3)：600-607,8.DOI:10.16178/j.issn.0528-9017.20240885
烟叶醇化过程中的差异微生物蛋白质及其来源分析
Analysis of differential microbial proteins and their sources during the aging process of tobacco leaves
摘要
Abstract
The microorganisms on the surface and inside of tobacco leaves are rich and diverse,which are closely related to the aging quality of tobacco leaves.In this study,the differential microbial proteins and source microorganisms of tobacco leaves with different aging time were analyzed by macroproteomics technology,and the enzymes related to improving the quality of tobacco leaves were excavated to identify the key functional microorganisms.The results showed that the microbial proteins of tobacco leaves with different aging time were significantly(p<0.05)different.During the aging process of tobacco leaves,Acinetobacter and Sphingomonas synergistically degraded tobacco cellulose through β-1,4-glucanase and β-glucosidase.Pseudomonas facilitated protein degradation in tobacco leaves by producing aminopeptidase,while Pseudomonas and Sphingomonas produced β-amylase and glucoamylase to degrade starch synergistically.In addition,dominant genera such as Pseudomonas,Acinetobacter,and Enterobacter were involved in the degradation of carotenoids and their derivatives.This study provides a theoretical basis for the subsequent improvement of tobacco leaves aging strategies.关键词
烟叶醇化/宏蛋白质组学/差异微生物蛋白质/功能微生物Key words
tobacco leaf aging/macroproteomic/differential microbial protein/functional microorganism分类
农业科技引用本文复制引用
张艺帆,胡宗玉,李锋,金立锋,杨月,附俊杰,王笑,许强,苗晨琳,陆震鸣..烟叶醇化过程中的差异微生物蛋白质及其来源分析[J].浙江农业科学,2026,67(3):600-607,8.基金项目
中国烟草总公司重点研发项目(110202102033) （110202102033）
