中国现代医生2026，Vol.64Issue(7)：6-11,6.DOI:10.3969/j.issn.1673-9701.2026.07.002
基于生物信息学筛选丹参酮ⅡA作用于心肌梗死的关键靶点
Based on bioinformatics,key targets of tanshinone ⅡA in the treatment of myocardial infarction were screened
摘要
Abstract
Objective Based on bioinformatics methods,the key targets of tanshinone ⅡA in treating myocardial infarction(MI)were systematically screened,and the molecular mechanism was explored.Methods The MI-related transcriptome data sets(GSE62646,GSE83500)were obtained from the Gene Expression Omnibus database,and integrate the TCMSP,CTD,and SwissTargetPrediction databases to obtain the potential targets of tanshinone ⅡA.The intersecting genes were extracted through Venn diagrams and a protein-protein interaction(PPI)network was constructed.Functional enrichment analysis,including Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analyses,was performed using DAVID.Molecular docking was conducted using CB-Dock2 to validate binding affinity.Results A total of 8 core candidate gene targets were identified,including SCN9A,PIM1,GSK3A,RELA,HMGB1,FASN,ACAT1 and MPI.Molecular docking showed that the binding energies of tanshinone ⅡA with all targets were lower than ˗6.8kcal/mol,with the strongest binding observed for SCN9A(˗10.6kcal/mol)and PIM1(˗10.2kcal/mol).GO analysis revealed that the targets were significantly enriched in biological processes such as inflammatory response and interleukin regulation.KEGG analysis indicated that the main pathways were fatty acid metabolism,the advanced glycation end product/advanced glycation end product receptor signaling pathway,and the acute myeloid leukemia pathway.Conclusion Tanshinone ⅡA may exert multi-dimensional protective effects in the treatment of MI by acting on multiple targets such as SCN9A and PIM1,and by synergistically regulating key pathways including inflammatory response and fatty acid metabolism.关键词
丹参酮ⅡA/心肌梗死/生物信息学/分子对接Key words
Tanshinone ⅡA/Myocardial infarction/Bioinformatics/Molecular docking分类
医药卫生引用本文复制引用
关仲宜,徐文华,郑景辉..基于生物信息学筛选丹参酮ⅡA作用于心肌梗死的关键靶点[J].中国现代医生,2026,64(7):6-11,6.基金项目
广西高校中青年教师科研基础能力提升项目(2022KY0269) （2022KY0269）
2023年国家级大学生创新创业训练计划项目(202310600031) （202310600031）
广西中医药大学青年基金项目(2021QN028) （2021QN028）
广西壮族自治区中医药管理局自筹经费科研课题(GXZYZ20210474) （GXZYZ20210474）
