中国医学工程2026，Vol.34Issue(2)：10-14,5.DOI:10.19338/j.issn.1672-2019.2026.02.003
DUSP10调控c-Fos/NFATc1信号通路抑制破骨细胞分化研究
DUSP10 regulates c-Fos/NFATc1 signaling pathway to suppress osteoclast differentiation
摘要
Abstract
[Objective]To investigate the regulatory role of DUSP10 in osteoclast differentiation and its molecular mechanism.[Methods]RAW264.7 cells were used as a model and divided into three groups:the NC group(untreated cells),the DUSP10-NC group(transfected with empty plasmid),and the DUSP10-OE group(transfected with DUSP10 overexpression plasmid),DUSP10-sh group(transfected with DUSP10 interference plasmid).Cell viability was assessed via CCK-8 assay,osteoclast differentiation was evaluated by TRAP staining,and protein expression levels of c-Fos,NFATc1,and TRAP were measured using Western blot.[Results]Overexpression of DUSP10 significantly inhibited osteoclast viability(P＜0.05),reduced the number of TRAP-positive cells(P＜0.05),and downregulated c-Fos,NFATc1,and TRAP protein expression(P＜0.005).Conversely,inhibition of DUSP10 promoted osteoclast differentiation(P＜0.05)and upregulated the expression of the aforementioned proteins.[Conclusion]DUSP10 suppresses osteoclast differentiation by inhibiting the c-Fos/NFATc1 signaling pathway,providing a novel theoretical basis for targeted therapies for bone metabolic disorders such as osteoporosis.关键词
DUSP10/破骨细胞/c-Fos/NFATc1/TRAPKey words
DUSP10/osteoclast/c-Fos/NFATc1/TRAP分类
医药卫生引用本文复制引用
刘梦想,陈磊,于洪涛..DUSP10调控c-Fos/NFATc1信号通路抑制破骨细胞分化研究[J].中国医学工程,2026,34(2):10-14,5.基金项目
新疆生产建设兵团科技计划资助(2023CB008-34) （2023CB008-34）
"天山英才"医药卫生高层次人才培养计划(CZ001222) （CZ001222）
石河子大学科研项目(ZZZC2023050) （ZZZC2023050）
石河子大学第一附属医院科技计划项目(QN202131) （QN202131）
