中医药信息2026，Vol.43Issue(3)：15-20,6.DOI:10.19656/j.cnki.1002-2406.20260303
基于PI3K/Akt信号通路探讨蠲浊调冲汤诱导细胞凋亡治疗子宫内膜息肉的作用机制
Investigation of the Mechanism of Action of Juanzhuo Tiaochong Decoction in Treating Endometrial Polyp by Inducing Apoptosis via the PI3K/Akt Signaling Pathway
摘要
Abstract
Objective To investigate the mechanism of action of Juanzhuo Tiaochong Decoction(JZTCT)in treating endometrial polyp(EP)through the induction of apoptosis via the PI3K/Akt signaling pathway.Methods Primary cells were isolated and cultured from surgical specimens of EP patients via enzymatic digestion,identified by immunofluorescence.Forty SD rats were randomly divided into four groups:blank control group,low-,medium-,and high-dose JZTCT groups,with 10 rats in each group.Rats in low-,medium-,and high-dose JZTCT groups were gavaged with JZTCT at the corresponding doses of 11.7,23.4,and 46.8 g/(kg·d)respectively,while rats in blank control group were gavaged with an equal volume of physiological saline.All groups were administrated twice daily for 7 consecutive days.After the final gavage,rats were fasted(with free access to water)for 12 h,and blood samples were collected from the abdominal aorta.EP cells were treated with the corresponding serum samples isolated,then the cells were divided into the following groups:blank group(EP primary cells+10%serum from the blank control group),low-dose group(EP primary cells+10%drug-containing serum from the low-dose JZTCT group),medium-dose group(EP primary cells+10%drug-containing serum from the medium-dose JZTCT group),and high-dose group(EP primary cells+10%drug-containing serum from the high-dose JZTCT group).Cell viability was assessed using the CCK-8 assay;Apoptosis rates were measured by flow cytometry;mRNA expression levels of PI3K and Akt were detected by qRT-PCR;Protein expression levels of apoptosis-related factors and key proteins in the PI3K/Akt pathway were evaluated by Western blot.Results Compared with the blank group,JZTCT-containing serum significantly inhibited the proliferation of EP primary cells and induced apoptosis,markedly suppressed the mRNA expression of PI3K and Akt,upregulated the protein expression of Bax and Caspase-3,and downregulated the expression of Bcl-2,p-PI3K/PI3K,and p-Akt/Akt(P<0.05).Conclusion JZTCT can exert its therapeutic effects on EP by inhibiting the PI3K/Akt signaling pathway and regulating downstream apoptosis-related factors.关键词
蠲浊调冲汤/子宫内膜息肉/细胞凋亡/PI3K/Akt信号通路Key words
Juanzhuo Tiaochong Decoction/Endometrial polyp/Apoptosis/PI3K/Akt signaling pathway引用本文复制引用
赵桢,刘芳媛,丁丹妮,韩凤娟..基于PI3K/Akt信号通路探讨蠲浊调冲汤诱导细胞凋亡治疗子宫内膜息肉的作用机制[J].中医药信息,2026,43(3):15-20,6.基金项目
中医药循证能力提升项目(国中医药科技中药便函[2023]24号) （国中医药科技中药便函[2023]24号）
黑龙江省中医药科研项目(ZYW2024-014) （ZYW2024-014）
