植物研究2026，Vol.46Issue(2)：195-208,14.DOI:10.7525/j.issn.1673-5102.2026.02.001
PtNF-Ys基因在油松花发育早期的表达与功能分析
Expression Characteristics and Functional Analysis of PtNF-Ys Gene during Early Flower Development in Pinus tabuliformis
摘要
Abstract
To elucidate the causes of the long juvenile phase and low seed yield in Pinus tabuliformis seed orchards and explore the underlying regulatory mechanisms,this study focused on the flowering-related genes during early stages of flower bud differentiation.The aim was to reveal the gene expression characteristics during this period and clarify the biological functions of the nuclear factor Y(NF-Y)family members.Transcriptome sequencing(RNA-seq)was performed on apical meristems collected from adult trees in the Wucheng National Seed Orchard of P.tabuliformis,Shanxi,China,from mid-July to mid-October.The results revealed that the expression levels of PtNF-YB8 and many other NF-Y genes increased significantly in mid-October.Multiple cis-elements,including gibberellin(GA)-responsive and light-responsive elements,were found within the PtNF-YB8 promoter.Furthermore,exogenous GA treatment of apical buds significantly up-regulated the expression level of PtNF-YB8.Subcellular localization assays in tobacco cells showed that the PtNF-YB8 protein was localized in both the cytoplasm and the nucleus.Heterologous overexpression of PtNF-YB8 in Arabidopsis resulted in a distinct delayed flowering phenotype.Yeast two-hybrid assays confirmed that PtNF-YB8 interacts with PtNF-YC9 and PtNF-YC10.In conclusion,PtNF-YB8 may function by forming complexes with NF-Y members,such as PtNF-YC9/10,to facilitate signal transduction and negatively regulate flowering time.These findings provide a theoretical basis for elucidating the flowering regulatory network in conifers and developing techniques for high and stable yields in seed orchards.关键词
油松/花芽发育/核因子家族/赤霉素Key words
Pinus tabuliformis/floral bud development/nuclear factor Y family/gibberellin分类
农业科技引用本文复制引用
丁文彬,胡岳涵,白寅甲,杨宇寒,薛华..PtNF-Ys基因在油松花发育早期的表达与功能分析[J].植物研究,2026,46(2):195-208,14.基金项目
国家重点研发计划项目(2023YFD2200605) （2023YFD2200605）
北京林业大学"5·5工程"科研创新团队项目(BLRC2023C06). （BLRC2023C06）
