植物研究2026，Vol.46Issue(2)：209-219,11.DOI:10.7525/j.issn.1673-5102.2026.02.002
兰香草花挥发性成分鉴定及其对访花昆虫行为的影响
Identification of Floral Volatiles from Caryopteris incana and Their Regulatory Effects on Floral Visitors Behavior
摘要
Abstract
The aim of this study was to identify the floral volatiles of Caryopteris incana and to explore their influence on the behavior of floral visitors.Flower color was measured with the Royal Horticultural Society Colour Chart(RHSCC),and floral volatiles were collected by adsorption-solvent desorption and identified by gas chromatography-mass spectrometry(GC-MS).Concurrently,insect visitation frequency was recorded through manual observations,and temporal patterns of pollen viability and stigma receptivity were assessed using the TTC(2,3,5-triphenyltetrazolium chloride)method and the benzidine-hydrogen peroxide assay,respectively.The results indicated that:(1)The flower color of C.incana was identified as strong violet(RHSCC N88B).(2)Terpenoids(77.25％)constituted the majority composition of floral volatiles in C.incana,followed by esters(13.00％).D-Limonene(36.80％)was the most abundant characteristic volatile.(3)Bees were the dominant floral visitors to C.incana,accounting for over 91％ of all visitors.Among them,Apis mellifera ligustica was the most frequent species,with a visitation frequency significantly higher than that of all other insects(P<0.001).(4)The flowering period of C.incana lasted about 7 days.Pollen remained viable on the 2nd day and 3rd day and stigma had receptivity on the 5th day,demonstrating a protandrous(male-first)dichogamy that effectively reduces the potential for self-pollination.This study contributes to a deeper understanding of the pollination ecology of C.incana.关键词
兰香草/花挥发性成分/访花昆虫/花粉活力/柱头可授性Key words
Caryopteris incana/floral volatiles/floral visitors/pollen viability/stigma receptivity分类
农业科技引用本文复制引用
何咏,乌芷静,张武凡,罗毅波,李睿思,王秋月,禹瑞敏,董树斌,程瑾..兰香草花挥发性成分鉴定及其对访花昆虫行为的影响[J].植物研究,2026,46(2):209-219,11.基金项目
中央高校基本科研业务费专项资金项目(BLX202317) （BLX202317）
战略科学家科技创新项目(KG20241031). （KG20241031）
