植物研究2026，Vol.46Issue(2)：259-269,11.DOI:10.7525/j.issn.1673-5102.2026.02.006
基于CRISPR/Cas9敲除阐明AtMST1通过H2S合成调控拟南芥耐盐性
The Role of AtMST1 in Regulating Salt Tolerance via H2S Synthesis in Arabidopsis Revealed by CRISPR/Cas9 Knockout
摘要
Abstract
Hydrogen sulfide(H2S)is an important gaseous signaling molecule in plants,and its biosynthesis relies on various endogenous enzymes.The enzyme 3-mercaptopyruvate sulfurtransferase(MST)has been established as an H2S generator in animal systems,and a similar enzymatic activity has been reported for Arabidopsis MST1.To further validate the H2S-producing function of AtMST1 in plants and explore its role in salt stress response,CRISPR/Cas9-mediated gene editing to target the AtMST1 gene was employed in Arabidopsis thaliana.By designing four target sites and constructing the gene editing vector,homozygous atmst1 mutants following Agrobacterium-mediated transformation were successfully obtained.Genotyping analysis revealed that the mutant carried a single T-nucleotide insertion at the target site,resulting in a frameshift mutation and premature termination of translation.Compared with the wild type,the atmst1 mutant showed a significant reduction in the intensity of H2S-specific fluorescent signals,as well as in both H2S content and production rate.Salt stress treatment resulted in a clear salt-sensitive phenotype and greater reactive oxygen species accumulation in the roots of atmst1 seedlings.In summary,this study successfully created an AtMST1 loss-of-function mutant,providing genetic evidence for its crucial role in endogenous H2S synthesis in plants and revealing its physiological function in positively regulating salt tolerance in Arabidopsis by modulating H2S levels.关键词
AtMST1基因/CRISPR/Cas9基因编辑技术/硫化氢/拟南芥/盐胁迫Key words
AtMST1 gene/CRISPR/Cas9 gene-editing technology/hydrogen sulfide/Arabidopsis thaliana/salt stress分类
生物科学引用本文复制引用
曹海艳,田楷文,贾晓宇,郝雪峰,金竹萍..基于CRISPR/Cas9敲除阐明AtMST1通过H2S合成调控拟南芥耐盐性[J].植物研究,2026,46(2):259-269,11.基金项目
山西省基础研究计划资助项目(202403021212063,202503021211222) （202403021212063,202503021211222）
山西大学杏花村学院开放基金项目(XCSXU-KF-202401). （XCSXU-KF-202401）
