中国中医骨伤科杂志2026，Vol.34Issue(4)：14-21,8.DOI:10.20085/j.cnki.issn1005-0205.260403
芍药苷对类风湿关节炎大鼠滑膜组织炎症的改善作用
The Ameliorative Effect of Paeoniflorin on Synovial Tissue Inflammation in Rats with Rheumatoid Arthritis
摘要
Abstract
Objective:To observe the effect and mechanism of paeoniflorin on synovial tissue in rats with rheumatoid arthri-tis(RA),focusing based on the PI3 K/Akt/NF-κB signaling pathway.Methods:Sixty male SD rats were randomly divided into the blank group,model group,PI3 K inhibitor group,paeoniflorin group,and paeoniflorin+PI3 K agonist group,with 12 rats in each group.Except for the blank group,the other groups were established with type Ⅱ collagen-induced rat rheumatoid arthritis models.Body weight and joint swelling degree of rats in each group were monitored at different time points;pathological changes in joint tissues were assessed by hematoxylin-eosin(HE)and Safranin O-Fast Green Stai-ning.The contents of TNF-α,IL-6,IL-1β and IL-10 in rat serum were detected by ELISA.The protein expressions of PI3 K,Akt,NF-κB p65,TNF-α,IL-6 and IL-10 in the synovial tissue of rats were detected by Western Blot.The mRNA expressions levels of PI3 K,Akt,NF-κB p65,TNF-α,IL-6 and IL-10 in rat synovial tissue were quantified by RT-PCR.Results:Compared with the model group,the body weight of the paeoniflorin+PI3 K agonist group increased and the de-gree of joint swelling decreased on the 21st and 28th day(P<0.05).The body weight of the paeoniflorin group and the PI3 K inhibitor group increased,and the degree of joint swelling was significantly reduced(P<0.01).Compared with the model group,the paeoniflorin+PI3 K agonist group exhibited mild articular cartilage injury and an increased number of chondrocytes.In the paeoniflorin group and the PI3 K inhibitor group,the articular cartilage injury was improved,and no obvious damage was observed in the subchondral bone structure.Compared with the model group,the levels of serum TNF-α,IL-6 and IL-1β in the paeoniflorin+PI3 K agonist group decreased,while the level of IL-10 increased(P<0.05),and the levels of TNF-α,IL-6 and IL-1β in the paeoniflorin group and the PI3 K inhibitor group significantly decreased(P<0.01).The level of IL-10 was significantly increased(P<0.01).Western Blot and RT-PCR showed that,compared with the model group,the paeoniflorin+PI3 K agonist group exhibited decreased protein expressions and gene levels of PI3 K,Akt,NF-κB p65,TNF-α,and IL-6 decreased(P<0.05),while the protein expression and gene level of IL-10 increased(P<0.05).The levels in the paeoniflorin group and the PI3 K inhibitor group changed significantly(P<0.0 1).Conclu-sion:Paeoniflorin can regulate the PI3 K/Akt/NF-κB signaling pathway,thereby improving synovial tissue inflammation in rats with rheumatoid arthritis.关键词
芍药苷/类风湿关节炎/滑膜组织/磷酸肌醇激酶/蛋白激酶/核转录因子-κBKey words
paeoniflorin/rheumatoid arthritis/synovial tissue/PI3 K/Akt/NF-κB分类
医药卫生引用本文复制引用
毛珍,江润,张涛,刘健,王龙..芍药苷对类风湿关节炎大鼠滑膜组织炎症的改善作用[J].中国中医骨伤科杂志,2026,34(4):14-21,8.基金项目
武汉市卫生计生科研基金资助项目(WZ24Q02)武汉市蔡甸区人民医院院内科研项目(XHJBYY-KXYJJJ-QNXM-05,XHJBKXYJJJ-MSXM-03) （WZ24Q02）
