植物研究2026，Vol.46Issue(2)：280-293,14.DOI:10.7525/j.issn.1673-5102.2026.02.008
复合诱导子和光质诱导子对箭叶淫羊藿悬浮细胞次生代谢产物的影响
Effects of Composite Inducers and Light Quality Inducers on Secondary Metabolites in Epimedium sagittatum Suspension Cells
摘要
Abstract
This study investigated the effects of composite inducers at varying concentrations and photomorphogenic inducers under different photoperiods on the major secondary metabolites in Epimedium sagittatum(Sieb.et Zucc.)Maxim.suspension cells.Orthogonal experiments were conducted under optimal culture conditions to evaluate the effects of three composite inducers:MeJA at 25,50,and 75 μmol·L-1;SA at 25,50,and 75 μmol·L-1;and CTS at 0,10,and 20 mg·L-1.Additionally,induction was performed under varying light qualities and photoperiods.The findings demonstrated that the levels of secondary metabolites,including flavonol glycosides,total flavonoids,total phenolic acids,and total saponins,increased in E.sagittatum suspension cells treated with composite inducers and photomorphogenic inducers compared to the control group.The F4 treatment group(50 μmol·L-1 MeJA,25 μmol·L-1 SA,20 mg·L-1 CTS)and G6 treatment group(blue light,16 h)exhibited significantly higher flavonol glycoside content,being 2.92 and 3.79-fold higher than the control,respectively.The F3 treatment group(25 μmol·L-1 MeJA,50 μmol·L-1 SA,10 mg·L-1 CTS)and G6 treatment group(blue light,16 h)demonstrated significantly higher total flavonoid content,approximately 1.42 and 1.27-fold higher than the control,respectively.The F2 treatment group(25 μmol·L-1 MeJA,25 μmol·L-1 SA)and G4 treatment group(blue light,12 h)exhibited significantly elevated total phenolic acid content,being 4.11 and 2.94-fold higher than the control,respectively.The F8 treatment group(75 μmol·L-1 MeJA,75 μmol·L-1 SA)and G2 treatment group(white light,12 h)exhibited higher total alkaloid content,being 1.28 and 1.02-fold higher than the control,respectively.The total saponin content in the F5 treatment group(50 μmol·L-1 MeJA,50 μmol·L-1 SA)and G6 treatment group(blue light,16 h)was significantly higher,being 3.73 and 3.05-fold higher than the control,respectively.These results indicated that the application of exogenous inducers effectively enhanced the synthesis and accumulation of secondary metabolites,such as flavonoids and total saponins,in E.sagittatum.关键词
箭叶淫羊藿/悬浮细胞/复合诱导子/光质诱导子/次生代谢产物Key words
Epimedium sagittatum/suspension cells/composite inducers/light quality inducers/secondary metabolites分类
农业科技引用本文复制引用
赵梦瑶,纪宝玉,李诺,韩耀祖,裴莉昕..复合诱导子和光质诱导子对箭叶淫羊藿悬浮细胞次生代谢产物的影响[J].植物研究,2026,46(2):280-293,14.基金项目
中央本级重大增减支项目(2060302) （2060302）
重庆市药物种植研究所2024年刘正宇全国名老中医药专家传承工作室开放课题(2024jbky-kfkt-007) （2024jbky-kfkt-007）
河南中医药大学博士科研基金(BSJJ2024-04) （BSJJ2024-04）
河南省现代农业产业技术体系项目(HARS-22-11-Z3). （HARS-22-11-Z3）
