中医药信息2026，Vol.43Issue(4)：24-29,6.DOI:10.19656/j.cnki.1002-2406.20260404
基于Nrf2/SLC7A11/GPX4通路探讨清解扶正颗粒诱导结直肠癌细胞铁死亡的实验研究
Study on the Induction of Ferroptosis in Colorectal Cancer Cells by Qingjie Fuzheng Granules through the Nrf2/SLC7A11/GPX4 Pathway
摘要
Abstract
Objective To investigate the in vitro mechanism of colorectal cancer(CRC)HCT-116 ferroptosis in cells induced by Qingjie Fuzheng Granules(QFG)through the signaling pathways involving Nuclear Factor E2-related Factor 2(Nrf2)/Solute Carrier Family 7 Member 11(SLC7A11)/Glutathione Peroxidase 4(GPX4).Methods Human colorectal cancer cell line HCT-116 was cultured in vitro and treated with different concentrations of QFG(0,0.05,0.10,0.25,0.50,0.75,1.00,1.50,and 2.00 mg/L).The inhibitory effect of QFG on cell proliferation was detected by CCK-8 method.According to the results,the doses of 0,0.5,1,and 2 mg/mL were designated as the control,low-,medium-,and high-dose groups respectively.Ultrastructural observations were performed via transmission electron microscopy;quantitative analysis of iron ion levels,reactive oxygen species(ROS),superoxide dismutase(SOD),malondialdehyde(MDA),and glutathione(GSH)were measured using specific kits.Western blot was employed to detect protein expression levels of Nrf2,SLC7A11,and GPX4.Results QFG exhibited dose-dependent inhibition of HCT-116 cell proliferation.Transmission electron microscopy revealed that after QFG treatment,cells exhibited typical morphological features of ferroptosis,such as shrunken mitochondria and ruptured cristae.QFG dose-dependently increased the levels of iron ions,ROS,and MDA(P<0.05,P<0.01),while decreased the activity of SOD and GSH(P<0.01).Meanwhile,QFG downregulated Nrf2,SLC7A11,and GPX4 protein expression(P<0.01),and this inhibitory effect was more pronounced with increasing concentrations.Conclusion QFG may induce ferroptosis in HCT-116 cells by inhibiting the Nrf2/SLC7A11/GPX4 signaling pathway,thereby exerting an anti-CRC effect.关键词
清解扶正颗粒/结直肠癌/铁死亡/Nrf2/SLC7A11/GPX4通路Key words
Qingjie Fuzheng Granules/Colorectal cancer/Ferroptosis/Nrf2/SLC7A11/GPX4 pathway引用本文复制引用
吴爱英,刘锦洪,贾沛芝,华杭菊,江颖秀,林久茂..基于Nrf2/SLC7A11/GPX4通路探讨清解扶正颗粒诱导结直肠癌细胞铁死亡的实验研究[J].中医药信息,2026,43(4):24-29,6.基金项目
福建省自然科学基金项目(2025J01901) （2025J01901）
2025年度福建省中青年教师教育科研项目(科技类)(JAT251322) （科技类）
漳州卫生职业学院2023年院级科研项目(ZWY202312) （ZWY202312）
