中国中医骨伤科杂志2026，Vol.34Issue(4)：30-35,6.DOI:10.20085/j.cnki.issn1005-0205.260405
水蛭素对糖尿病足溃疡大鼠创面愈合及血管生成的影响
Effects of Hirudin on Wound Healing and Angiogenesis of Diabetic Foot Ulcer Rats
摘要
Abstract
Objective:To investigate the effects of hirudin on wound healing and angiogenesis in diabetic foot ulcer(DFU)rats through regulation of the vascular endothelial growth factor(VEGF)/Notch1 pathway.Methods:The DFU rats were randomly divided into the following groups:control group,model group,low-dose hirudin group,high-dose hirudin group,and inhibitor group,with 10 rats in each group.The wound healing rate of rats in each group was measured.ELISA was performed to measure the expression levels of TNF-α,IL-8,and IL-6 in serum.HE staining was performed to assess path-ological changes in rat wound tissue.Immunohistochemical staining was performed to detect CD34 expression and micro-vascular density(MVD)in wound tissue.Moreover,Western Blot was used to measure the expression levels of VEGF and Notch1 proteins in rat wound tissues.Results:The skin tissue structure of the control group was normal and showed no pathological changes.The distribution of new blood vessels in the model group was relatively sparse,and with a large amount of inflammatory cell infiltration.In the low-dose and high-dose thrombin groups,the number of new blood vessels increased successively,while the inflammatory cell infiltration decreased successively.The inhibitor group showed a fur-ther reduction in neovascularization and an increase in inflammatory cell infiltration.The model group had higher levels of TNF-α,IL-8,and IL-6,and lower wound healing rate,CD34 positivity rate,MVD,expression of VEGF and Notch1 than the control group(P<0.05).The low-dose hirudin and high-dose hirudin groups had lower TNF-α,IL-8,and IL-6,and higher wound healing rates,CD34 positivity rates,MVD,VEGF,and Notch1 than the model group(P<0.05).The inhibitor group had higher levels of TNF-α,IL-8,and IL-6,and lower wound healing rate,CD34 positivity rate,MVD,expression of VEGF and Notch1 compared to the high-dose hirudin group(P<0.05).Conclusion:Hirudin may promote wound healing and angiogenesis in DFU rats by activating the VEGF/Notch1 pathway.关键词
水蛭素/血管内皮生长因子/缺刻基因1/糖尿病足溃疡/创面愈合/血管生成Key words
hirudin/vascular endothelial growth factor/Notch1/diabetic foot ulcer/wound healing/angiogenesis分类
医药卫生引用本文复制引用
董洁,王丽娟,孙艳红,刘昊..水蛭素对糖尿病足溃疡大鼠创面愈合及血管生成的影响[J].中国中医骨伤科杂志,2026,34(4):30-35,6.基金项目
河北省中医药类科学研究课题计划项目(2025521) （2025521）
