中医药信息2026，Vol.43Issue(4)：30-37,8.DOI:10.19656/j.cnki.1002-2406.20260405
泽兰合剂对膝骨关节炎模型膝关节损伤后滑膜炎症反应及凝血功能的作用机制研究
Study on Mechanism of Action of Zelan Mixture in Regulating Synovial Inflammatory Response and Coagulation Function after Knee Joint Injury in Knee Osteoarthritis Model
摘要
Abstract
Objective To explore the molecular mechanism of Zelan Mixture in intervening in the stress response in knee osteoarthritis(KOA)model rats from the perspectives of inflammatory response and coagulation function.Methods Fifty-five SD rats were randomly divided into the control group,the model group,and the low-,medium-,and high-dose Zelan Mixture groups.In all groups except for the control group,KOA animal model was built by anterior cruciate ligament transection method.After successful modeling,the control group and the model group were given equivalent volume of normal saline by gavage,while the low-,medium-,and high-dose Zelan Mixture groups were given 1 mL,2 mL,and 4 mL Zelan Mixture by gavage,respectively.The administration was performed twice a day for 7 days.The abdominal aortic blood and knee synovium of SD rats in each group were collected on the first,third and seventh day after gavage intervention for relevant tests.HE staining was used to observe the condition of synovial tissue in SD rats;Synovial tissue morphology score were used to evaluate the morphological change of synovial tissue;RT-qPCR was used to detect the relative expression of inflammatory-related genes IL-6,TNF-α,and NF-κB p65 in synovial tissue of SD rats in each group;Western blot was used to detect the relative expression of inflammatory-related proteins IL-6,TNF-α,and NF-κB p65 in synovial tissue of SD rats in each group;ELISA was used to detect the content of IL-6,TNF-α,ICAM,VCAM,and TXA2 in the serum of SD rats in each group.Results ①Synovial tissue morphology score:Compared with the model group,the synovial tissue morphology score of the high-dose Zelan Mixture group was significantly lower(P<0.05);②RT-qPCR:Compared with the model group,the relative expression levels of IL-6,TNF-α,and NF-κB p65 mRNA in synovial tissue of the medium-and high-dose Zelan Mixture groups were significantly lower(P<0.01);③Western blot:Compared with the model group,the relative expression levels of IL-6,TNF-α,and NF-κB p65 protein in synovial tissue of the medium-and high-dose Zelan Mixture groups were significantly lower(P<0.05);④ELISA:Compared with the model group,the serum contents of IL-6,TNF-α,ICAM,VCAM,and TXA2 in the low-,medium-,and high-dose Zelan Mixture groups were significantly lower at seventh day after surgery(P<0.05).Conclusion Zelan Mixture can alleviate the inflammatory indicators and improve the hypercoagulable state in KOA model rats,regulate the related expression of inflammatory-related genes and proteins in synovial tissue,and regulate the levels of inflammatory factors and coagulation factors in serum,thereby ameliorating the stress response in KOA model rats.The mechanism of action may be related to the reduction of inflammatory response and improvement of hypercoagulable state.关键词
泽兰合剂/KOA模型大鼠/膝骨关节炎/应激反应/凝血功能/作用机制Key words
Zelan Mixture/KOA model rats/Knee osteoarthritis/Stress response/Coagulation function/Mechanism of action引用本文复制引用
郭英,刘金清,明雄举,申震,艾元亮,许燕飞,单彭招..泽兰合剂对膝骨关节炎模型膝关节损伤后滑膜炎症反应及凝血功能的作用机制研究[J].中医药信息,2026,43(4):30-37,8.基金项目
云南省科技厅科技计划项目(202301AZ070001-087,202101AZ070001-257) （202301AZ070001-087,202101AZ070001-257）
云南省"高层次人才培养支持计划"项目(YNWR-MY-2020-074) （YNWR-MY-2020-074）
