中医药信息2026，Vol.43Issue(4)：38-43,6.DOI:10.19656/j.cnki.1002-2406.20260406
基于方证对应探讨凉血消风散干预特应性皮炎风湿热蕴证的机制
Exploration on Mechanism of Liangxue Xiaofeng Powder in Intervening Atopic Dermatitis with Wind-Damp-Heat Accumulation Pattern Based on Formula-Pattern Correspondence
摘要
Abstract
Objective To explore the mechanism of action of Liangxue Xiaofeng Powder in treating atopic dermatitis(AD)with wind-damp-heat accumulation pattern,with a focus on analyzing its dose-dependent effect on regulating Th1/Th2 immune balance and serum inflammatory factors from the perspective of formula-pattern correspondence.Methods A total of 30 SD rats were divided into control group,atopic dermatitis with wind-damp-heat accumulation pattern group(AD-JB group),low-dose Liangxue Xiaofeng Powder group(LXXFS-L group),medium-dose Liangxue Xiaofeng Powder group(LXXFS-M group)and high-dose Liangxue Xiaofeng Powder group(LXXFS-H group).Excluding the control group,all other groups were administered with a 7%dinitrochlorobenzene(DNCB)acetone solution to induce the atopic dermatitis model,and received wind-damp-heat stimulation simultaneously for 14 days.On the 15th day of modeling,LXXFS-L,M,and H groups were administered Liangxue Xiaofeng Powder at doses of 1.4,2.8,and 5.6 g/mL by gavage respectively,while control group and AD-JB group were given an equal volume of normal saline by gavage respectively.The gavage lasted for 14 consecutive days.After the intervention,serum levels of cytokines IL-4,IL-6,and immunoglobulin IgE of rats in each group were detected;The lesional skin on the back of rats was collected and processed into pathological sections for HE staining and immunofluorescence detection.Results ①Dose-dependent inhibition of inflammatory factors(detected by ELISA)was observed:serum levels of cytokines IL-4,IL-6,and immunoglobulin IgE reduced significantly in the LXXFS-H group(P<0.01).②There was a dose-dependent restoration of immune balance(detected by immunofluorescence):the expression ratio of INF-γ/IL-4 was relatively low in the AD-JB group,while the ratio of INF-γ/IL-4 in the LXXFS-H group was significantly higher than that in the AD-JB group.③The therapeutic effect showed a gradient of LXXFS-L group<LXXFS-M group<LXXFS-H group.Conclusion Liangxue Xiaofeng Powder exerts a stepped therapeutic effect on AD with wind-damp-heat accumulation pattern,by dose-dependently regulating Th1/Th2 immune imbalance and the release of serum inflammatory factors.This provides experimental evidence for the TCM theory of"formula-pattern correspondence"in guiding individualized medication.关键词
凉血消风散/特应性皮炎/风湿热蕴证/Th1/Th2免疫平衡/方证对应Key words
Liangxue Xiaofeng Powder/Atopic dermatitis/Wind-damp-heat accumulation pattern/Th1/Th2 immune balance/Formula-pattern correspondence引用本文复制引用
张磊,周朝旺,钟汝钱,李鑫,钟燕,黎妍,黄成,王建国..基于方证对应探讨凉血消风散干预特应性皮炎风湿热蕴证的机制[J].中医药信息,2026,43(4):38-43,6.基金项目
湖南省中医药管理局项目(C2023015) （C2023015）
湖南省教育厅重点项目(22A0280) （22A0280）
