肿瘤防治研究2026，Vol.53Issue(3)：187-193,7.DOI:10.3971/j.issn.1000-8578.2026.25.0712
乳腺癌细胞LncRNA OIP5-AS1对巨噬细胞极化的影响以及极化巨噬细胞对乳腺癌细胞的反馈调节
Effect of LncRNA OIP5-AS1 in Breast Cancer Cells on Macrophage Polarization and Feedback Regulation of Polarized Macrophages on Breast Cancer Cells
摘要
Abstract
Objective To explore the mechanism by which breast cancer-derived LncRNA OIP5-AS1 regulates the migration,invasion,and epithelial-mesenchymal transition of breast cancer cells through the M2 polarization of tumor-associated macrophages(TAM).Methods MDA-MB-231 cells were divided into the control group(blank control),the NC group(transfected with NC siRNA),and the si-OIP5 group(transfected with LncRNA OIP5-AS1 siRNA).The mRNA expression levels of LncRNAs OIP5-AS1,IL-4,and IL-13 were detected by RT-qPCR.The protein expression levels of IL-4 and IL-13 in the culture supernatant were detected by ELISA.The culture supernatant from the control group was added to RPMI 1 640 medium at different volume ratios to induce the polarization of M0 macrophages into TAMs.The mRNA expression of CD206 in TAMs was detected by RT-qPCR.M0-type macrophages were induced with the medium supernatant of the NC group/si-OIP5 group,and the expression of CD206 was detected by Western blot.Subsequently,a co-culture model of macrophage and MDA-MB-231 was constructed to evaluate the migration and invasion abilities of MDA-MB-231 cells and detect the expression changes in Vimentin,N-cadherin and E-cadherin.Results Compared with the control and NC groups,the si-OIP5 group showed significantly downregulated expression of LncRNA OIP5-AS1(P<0.001)and significantly decreased IL-13 mRNA and protein levels(P<0.05).Meanwhile,no significant difference was observed in the expression of IL-4 among the groups.Conditioned medium induced CD206 expression in M0 macrophages in a volume-dependent manner,with the strongest effect at a 40%volume ratio(P<0.001).The CD206 protein level in the si-OIP5 group significantly decreased(P<0.01).In the co-culture system,the migration and invasion abilities of MDA-MB-231 cells in the si-OIP5 group significantly weakened(P<0.001),the expression of E-cadherin was upregulated,and the expression levels of N-cadherin and Vimentin were downregulated(both P<0.01).Conclusion Breast cancer-derived LncRNA OIP5-AS1 can induce TAM M2 polarization mediated by IL-13,thereby promoting the migration,invasion,and EMT of breast cancer cells.关键词
乳腺癌/M2型巨噬细胞/LncRNA OIP5-AS1/迁移/上皮间质转化Key words
Breast neoplasms/M2 macrophages/LncRNA OIP5-AS1/Migration/Epithelium-mesenchymal transformation分类
医药卫生引用本文复制引用
杨恩帅,董哲,常鑫悦,肖子阳,刘洋,郭素芬..乳腺癌细胞LncRNA OIP5-AS1对巨噬细胞极化的影响以及极化巨噬细胞对乳腺癌细胞的反馈调节[J].肿瘤防治研究,2026,53(3):187-193,7.基金项目
Doctoral Research Startup Fund of Mudanjiang Medical University(No.2021-MYBSKY-012) （No.2021-MYBSKY-012）
Natural Science Foundation of Heilongjiang Province of China(No.LH2019H199) 牡丹江医科大学博士启动基金(2021-MYBSKY-012) （No.LH2019H199）
黑龙江省自然科学基金(LH2019H199) （LH2019H199）
