中华耳科学杂志2026，Vol.24Issue(4)：338-346,9.DOI:10.3969/j.issn.1672-2922.2026.04.009
地塞米松通过抑制内耳炎症干预噪声性聋的机制研究
Dexamethasone alleviates noise-induced hearing loss through inhibition of inner ear inflammation
摘要
Abstract
Objective To investigate whether dexamethasone has a protective effect against noise-induced hearing loss and its molecular pathological mechanisms.Methods Five-week-old male C57BL/6J mice were exposed to 100 dB SPL white noise for 2 hours,followed by a second noise exposure(NE)under the same conditions 14 days after the 1st NE to establish a mice model of noise-induced hearing loss.Prior to the 1st NE,dexamethasone was administered via intraperitoneal injection for 7 consecutive days.The effects of dexamethasone on noise-induced hearing loss were evaluated using auditory brainstem response(ABR)audiometry,immunofluorescence staining,ELISA,and Q-PCR.Results The number of ribbon synapses in the cochlea after NE significantly decreased(P<0.01),with a gradual increase of the level of inflammatory cytokine IL-18 in the inner ear(P<0.01).ELISA and Q-PCR analyses demonstrated downregulated expression of inner ear inflammatory cytokine IL-18 after NE with intraperitoneal injection of dexamethasone(P<0.01).Immunofluorescence staining showed significantly higher number of ribbon synapses with dexamethasone treatment than with saline intervention after NE,particularly after the 2nd NE(P<0.05).ABRs indicated significantly better thresholds with dexamethasone treatment at 1 day(4,16 and 24 kHz)and 7 days(4 and 16 kHz)after the 1st NE(P<0.05),and at 1 day(4 and 24 kHz),7 days(4,8 and 24 kHz),and 14 days(4,8 and 16 kHz)after the 2nd NE(P<0.05).Conclusions Dexamethasone can protect cochlear ribbon synapses against NE through inhibition of inner ear inflammation,providing a new insight into therapeutic strategy for noise-induced hearing loss.关键词
噪声暴露/耳蜗带状突触/地塞米松/内耳炎症/噪声性听力损失Key words
noise exposure/cochlear ribbon synapses/dexamethasone/inner ear inflammation/noise-induced hearing loss引用本文复制引用
郭瑞,徐义策,时晰,龚树生,柳柯..地塞米松通过抑制内耳炎症干预噪声性聋的机制研究[J].中华耳科学杂志,2026,24(4):338-346,9.基金项目
北京市科委-教委联合基金项目(KZ20231002542) （KZ20231002542）
首都医学科学创新中心科研培育项目资助(CX24PY14) （CX24PY14）
国家自然科学基金项目(82371151,82071037,82301298) （82371151,82071037,82301298）
2023年度"友谊种子计划"人才项目(YYZZ202326) （YYZZ202326）
孝感市自然科学计划项目(XGKJ2023010010) （XGKJ2023010010）
