作物研究2026，Vol.40Issue(1)：33-40,8.
酶菌复配和青贮天数对五节芒青贮品质的影响
Effects of Enzyme-Microbial Complex and Ensiling Duration on the Silage Quality of Miscanthus floridulus
摘要
Abstract
To investigate the effects of cellulase combined with different fermentation types of lactic acid bacteria and fermentation days on the silage quality of Miscanthus floridulus,and to screen the optimal enzyme-bacteria combination and fermentation period,this experiment used M.floridulus at the flowering stage as raw material for silage.Five treatments were set up:cellulase(EX),cellulase+Lactobacillus acidophilus(EX+LA),cellulase+Lactobacillus buchneri(EX+LB),cellulase+Lactobacillus plantarum(EX+LP),and a distilled water control group(CK).The fermentation quality and nutritional quality of each treatment were determined after 30 and 50 days of ensiling,respectively.The grey correlation analysis method was used for the comprehensive evaluation of the silage quality.The results showed that the pH and the ratio of ammoniacal nitrogen to total nitrogen in all enzyme microbial treatment groups were significantly lower than those in the CK group.The EX+LP group had the highest lactic acid content(30 d:1.71 mg/mL;50 d:1.66 mg/mL),while the EX+LB group had the highest acetic acid content(30 d:0.84 mg/mL;50 d:1.03 mg/mL).The neutral detergent fiber content in all additive treatment groups was significantly lower than that in the CK group,and the EX group had the highest crude protein content(30 d:72.0 g/kg;50 d:72.8 g/kg).After 30 days of ensiling,the EX treatment was identified as the optimal scheme(correlation degree 0.990);the EX+LB group showed the poorest comprehensive performance due to its high acid detergent lignin content and low lactic acid content.Comprehensive analysis indicates that after 30 days of ensiling,treatments with cellulase alone or its combination with L.acidophilus can improve the nutritional quality during the fermentation period,while its combination with L.plantarum can optimize the fermentation quality.关键词
五节芒/青贮/发酵/纤维素酶/乳酸菌Key words
Miscanthus floridulus/silage/fermentation/cellulase/lactic acid bacteria分类
农业科技引用本文复制引用
崔晨阳,石雪云,汪雅婷,曹欣,贺海洋,刘正君,张志飞..酶菌复配和青贮天数对五节芒青贮品质的影响[J].作物研究,2026,40(1):33-40,8.基金项目
国家重点研发计划(2024YFD1301204) （2024YFD1301204）
湖南省中央引导地方科技发展资金项目(2024ZYQ085). （2024ZYQ085）
