癌变·畸变·突变2026，Vol.38Issue(2)：113-118,6.DOI:10.3969/j.issn.1004-616x.2026.02.005
四名健康志愿者外周血总RNA辐射敏感分子的筛选
Screening for radiation-responsive molecules in blood RNA from four healthy volunteers
摘要
Abstract
OBJECTIVE:To investigate changes in differentially expressed genes in peripheral blood after in vitro exposure to different doses of 60Co γ rays and to identify new biomarkers for radiation damage.METHODS:Peripheral blood from four healthy adult volunteers were irradiated with different doses of 60Co γrays(0,2,4,6,and 8 Gy).Total RNA samples were extracted from irradiated blood using RNA high-throughput sequencing(RNA seq).The sequencing data was analyzed using bioinformatics methods.The R language software was used to screen differentially expressed genes between groups,and a common set of differentially expressed genes was identified.In addition,GO functional enrichment analysis and KEGG pathway enrichment analysis were performed on the differentially expressed genes using the Metascape platform.A protein interaction network was constructed using STRING database and key genes were screened using the Cytoscape software.RESULTS:A total of 281 common and differentially expressed genes were screened,including 89 lncRNAs and 192 mRNAs.The enrichment of these expressed genes was shown by GO to mainly included lymphocyte activation,positive regulation of immune effector process,positive regulation of immune response,inflammatory response,regulation of cellular component size.The KEGG pathway was enriched in cytokine cytokine receptor interactions,viral carcinogenesis,Th17 cell differentiation,ribosomes,T cell receptor signaling pathways,platelet activation,and intercellular adhesion junctions.Protein-protein interaction network screening identified 10 key genes:CD80,CD40,IL2RA,CCR7,NFKB1,TP53,IL21R,STAT6,PDCD1,and SLAMF1.CONCLUSION:The 10 key genes might be useful biomarkers for demonstration of radiation damage.关键词
电离辐射/RNA测序/辐射损伤/生物标志物Key words
ionizing radiation/RNA sequencing/radiation damage/biomarkers分类
能源科技引用本文复制引用
张成思,姜红,龙高群,李远航,田祝娟..四名健康志愿者外周血总RNA辐射敏感分子的筛选[J].癌变·畸变·突变,2026,38(2):113-118,6.基金项目
贵州省卫生健康委科学技术基金(gzwkj2024-482) （gzwkj2024-482）
贵州省疾病预防控制中心青年科学基金(2024-E-5) （2024-E-5）
