草业科学2026，Vol.43Issue(2)：361-369,9.DOI:10.11829/j.issn.1001-0629.2025-0022
前处理和胚切割方式对小黑麦成熟胚愈伤组织培养的影响
Effects of pretreatment and embryo-cutting method on callus culture of mature triticale embryos
摘要
Abstract
Mature triticale embryos were used as explants to investigate the effects of different disinfection,embryo-cutting and seed-soaking methods on callus induction and sprout growth.Various concentrations of ethanol and sodium hypochlorite solution were used for different durations for disinfecting triticale seeds.The callus induction frequency was higher and the rate of bacterial contamination was lower than those in the control when applying 75%ethanol for 1 min and 20%sodium hypochlorite solution for 15 min for disinfecting triticale callus,which was the most effective pretreatment disinfection method.Soaking seeds with 1 g·L-1 2,4-D or picloram solution did not increase the frequency of callus induction but markedly reduced the sprout rate.Transecting two-thirds of the embryos considerably increased the callus induction frequency.Combining this transection with soaking the seeds in 1 g·L-1 2,4-D was found to be an effective preinduction treatment.These results provide technical support for tissue culture and developing triticale germplasm resources.关键词
小黑麦/愈伤组织培养/种子消毒/切胚方式/浸种Key words
triticale/callus culture/seed disinfection/embryo cutting/soaking of seeds引用本文复制引用
杨东明,杨国栋,袁小淞,魏兴勇,王佺珍,高景慧,闫明科..前处理和胚切割方式对小黑麦成熟胚愈伤组织培养的影响[J].草业科学,2026,43(2):361-369,9.基金项目
国家自然科学基金项目(32301486) （32301486）
