草业科学2026，Vol.43Issue(2)：457-472,16.DOI:10.11829/j.issn.1001-0629.2024-0692
不同水平苜蓿叶粉对妊娠母猪粪便体外发酵特性及菌群结构的调控作用
Regulatory effects of different levels of alfalfa leaf meal on in vitro fermentation characteristics and microbial community structure in feces from gestating sows
摘要
Abstract
In this study,an in vitro fermentation model was constructed using fecal microbiota from gestating sows as the inoculum to investigate the effects of different levels of alfalfa leaf meal(ALM)on gas production,short-chain fatty acids(SCFAs)production,and microbial community composition under different fermentation times.This approach aims to optimize feed formulation to improve sow intestinal health and reproductive performance,reduce environmental pollution,and enhance economic benefits.Fecal microbiota from healthy gestating sows were used as the inoculum,and ALM was selected as the fermentation substrate.Four ALM levels were applied:0 mg(CON),50 mg(LALM),100 mg(MALM),and 200 mg(HALM).Fermentation broth and substrate residues were collected at 8,12,24,and 36 h for the determination of gas production,SCFAs yield,and microbiota composition.The results showed that total gas production and total SCFAs content in the fermentation broth at each time point tended to increase with increasing ALM levels.The addition of ALM significantly reduced H2S production(P<0.05).Acetic acid content increased significantly(P<0.01)following ALM addition,while valeric acid,isobutyric acid,and isovaleric acid contents were significantly increased(P<0.01)after 12 h of fermentation.Compared with the control group,the relative abundance of Fusobacterium was significantly reduced(P<0.05),whereas the relative abundance of Collinsella,Prevotellaceae_NK3B31_group,and Actinobacteriota was significantly increased(P<0.05)following ALM addition.The relative abundances of Prevotella,Olsenella,and Holdemanella exhibited significant positive correlations with fermentation gas production and short-chain fatty acids,while the former two genera showed highly significant negative correlations with H2S(P<0.01).In summary,supplementation with ALM in the in vitro fermentation of gestating sow fecal microbiota improved microbial community structure,promoted SCFAs production,and increased H2,CH4 and CO2 production.关键词
妊娠母猪/体外发酵/短链脂肪酸/产气/微生物菌落结构/苜蓿叶粉/相关性Key words
gestating sows/in vitro fermentation/short-chain fatty acids/gas production/microbial community structure/alfalfa leaf meal/correlation引用本文复制引用
程佳雯,张舒航,韩尧,皇甫卫康,刘孟琦,刘伯帅,崔亚垒,王志昌,史莹华..不同水平苜蓿叶粉对妊娠母猪粪便体外发酵特性及菌群结构的调控作用[J].草业科学,2026,43(2):457-472,16.基金项目
国家现代农业产业技术体系项目(CARS-34) （CARS-34）
中原科技创新领军人才项目(244200510010) （244200510010）
河南省科技攻关(242102110055) （242102110055）
