吉林医药学院学报2026，Vol.47Issue(2)：96-102,7.
预知子提取物的不同分离部位对脂多糖诱导BV2细胞介导神经炎症作用及其机制的研究
Mechanism of Action of Different Isolated Parts of Akebiae Fructus Extract on LPS-Induced Neuro-Inflammation in BV2 Cells
摘要
Abstract
Objective To investigate the anti-neuroinflammatory effects of different fractions of the Akebiae Fructus extract on lipopoly-saccharide(LPS)-induced BV2 cells and to explore the underlying mechanisms.Methods The Akebiae Fructus extract(JE)was ob-tained by ethanol reflux extraction,and the systematic solvent extraction method was used to separate the n-hexane fraction(JE1),di-chloromethane fraction(JE2),ethyl acetate fraction(JE3),and n-butanol fraction(JE4).An inflammatory model was established using LPS-induced BV2 cells;MTT assay was used to observe the cell viability.The content of NO in cell supernatant was measured by Griess reagent.The levels of PGE2,IL-6 and TNF-α were detected by ELISA kits.Detection of COX-2、iNOS protein and MAPK、NF-κB sig-naling pathway related protein expression levels using Western Blot method.Results Both JE1 and JE2 significantly reduced the levels of NO,PGE2,IL-6,TNF-α and the protein expressions of COX-2 and iNOS in LPS-induced BV2 cells,and also significantly decreased the protein expressions of P38,ERK,JNK,and NF-κB.Conclusion The JE1 and JE2 fractions of the Akebiae Fructus extract can effec-tively prevent LPS-induced neuroinflammatory responses occur in BV2 cells,and the underlying mechanism is associated with the inhibi-tion of the inflammatory NF-κB and MAPK signaling pathways.关键词
预知子提取物/脂多糖/BV2细胞/神经炎症Key words
Akebiae Fructus extract/lipopolysaccharide/BV2 cells/neuro-inflammation分类
医药卫生引用本文复制引用
陈欢,查红群,尹增盛,李静,刘文波,凌云,金洪光..预知子提取物的不同分离部位对脂多糖诱导BV2细胞介导神经炎症作用及其机制的研究[J].吉林医药学院学报,2026,47(2):96-102,7.基金项目
国家自然科学基金项目(31660104) （31660104）
江西省中医药管理局科技计划-重点项目(2021Z017) （2021Z017）
江西省科技厅自然科学基金面上项目(20224BAB206095) （20224BAB206095）
