广西医学2026，Vol.48Issue(3)：403-409,7.DOI:10.11675/j.issn.0253-4304.2026.03.15
急性高原环境下大鼠肠道屏障功能损伤与血液及尿液肠型脂肪酸结合蛋白水平的相关性研究
Correlation between intestinal barrier dysfunction and levels of intestinal fatty acid binding protein in blood and urine of rats under acute high altitude environment:a research study
摘要
Abstract
Objective To investigate the effects of different altitudes on intestinal barrier function in rats,and to analyze the correlation between intestinal fatty acid binding protein(I-FABP)levels in blood and urine and intestinal barrier dysfunction.Methods Eighteen healthy male SD rats were randomly divided into control group,3500 m plateau group,or 5000 m plateau group,with 6 rats in each group.A rat model of acute high-altitude intestinal barrier dysfunction was established.The structural changes of jejunal villi in the three groups were observed using light and electron microscopy.The jejunal villus height and crypt depth were measured and compared between rats in the three groups.Western blot was used to detect and compare the expressions of Claudin-1,Occludin,and zonula occludens 1(ZO-1)in jejunal tissue.ELISA was used to measure and compare serum levels of intestinal permeability markers including diamine oxidase(DAO),D-lactate acid(D-LA),lipopolysaccharide(LPS)and I-FABP levels in serum and urine.Pearson correlation analysis was performed to assess the correlation between serum/urine I-FABP levels and intestinal barrier dysfunction-associated indices.Results Light and scanning electron microscopy revealed that structural damage to jejunal villi in rats from both the 3500 m and 5000 m plateau groups,characterized by shortened villus height and shallower crypts.Compared to the control group,the 3500 m and 5000 m plateau groups showed decreased jejunal villus height,crypt depth,and expressions of Claudin-1,Occludin,and ZO-1 in jejunal tissue;in addition,these decreases were more pronounced in the 5000 m plateau group compared to the 3500 m plateau group(P<0.05).Serum levels of DAO,D-LA,LPS,I-FABP,and urine I-FABP level were higher in both 3500 m and 5000 m plateau groups compared to the control group,with the 5000 m plateau group showing higher levels than the 3500 m plateau group(P<0.05).Correlation analysis indicated that serum I-FABP level positively correlated with serum DAO and LPS levels(P<0.05).Urine I-FABP level positively correlated with serum I-FABP,DAO,LPS,and D-LA levels(P<0.05).Both serum and urine I-FABP level negatively correlated with the expressions of Claudin-1,Occludin,and ZO-1 in jejunal tissue(P<0.05).Conclusion Acute high-altitude exposure can lead to intestinal barrier dysfunction in rats,manifested as pathological damage to jejunal villi structure,decreased expression of tight junction proteins,and increased permeability.Serum and urine I-FABP levels positively correlates with intestinal barrier dysfunction,suggesting that I-FABP is an important biomarker reflecting intestinal mucosal injury.关键词
急性高原环境/肠道屏障功能/肠型脂肪酸结合蛋白/肠道通透性/紧密连接蛋白/大鼠/动物实验Key words
Acute high-altitude environment/Intestinal barrier function/Intestinal fatty acid binding protein/Intestinal permeability/Tight junction protein/Rats/Animal experiment分类
医药卫生引用本文复制引用
艾阳,李雨溪,张杰,钟林灿,聂瑚玮,杨文生,张林..急性高原环境下大鼠肠道屏障功能损伤与血液及尿液肠型脂肪酸结合蛋白水平的相关性研究[J].广西医学,2026,48(3):403-409,7.基金项目
四川省中央引导地方科技发展专项项目(2022ZYD0063) （2022ZYD0063）
